Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-cyclopentadione | 1.0 mM | Mucor ambiguus | |
3-methyl-1,2-cyclohexadione | 1.0 mM | Mucor ambiguus | |
5,5'-dithiobis(2-nitrobenzoic acid) | 0.1 mM | Mucor ambiguus | |
8-hydroxyquinoline | 0.1 mM | Mucor ambiguus | |
CdCl2 | 1.0 mM | Mucor ambiguus | |
cyclohexadione | 1.0 mM | Mucor ambiguus | |
dicoumarol | 0.1 mM | Mucor ambiguus | |
FeCl2 | 1.0 mM | Mucor ambiguus | |
additional information | overview | Mucor ambiguus | |
NADP+ | 1.0 mM | Mucor ambiguus | |
o-phenanthroline | 1.0 mM | Mucor ambiguus | |
quercetin | 0.01 mM | Mucor ambiguus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0123 | - |
1-methylisatin | - |
Mucor ambiguus | |
0.05 | - |
isatin | - |
Mucor ambiguus | |
0.053 | - |
beta-naphthoquinone | - |
Mucor ambiguus | |
0.135 | - |
NADPH | - |
Mucor ambiguus | |
0.21 | - |
acenaphthenequinone | - |
Mucor ambiguus | |
0.24 | - |
dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione | - |
Mucor ambiguus | |
0.38 | - |
dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione | - |
Mucor ambiguus | |
0.41 | - |
p-toluquinone | - |
Mucor ambiguus | |
0.58 | - |
dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione | - |
Mucor ambiguus | |
0.71 | - |
ketopantoyl lactone | - |
Mucor ambiguus | |
1.61 | - |
dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione | - |
Mucor ambiguus | |
2.5 | - |
alloxan | - |
Mucor ambiguus | |
3.3 | - |
dihydro-4-methyl-4-propyl-2,3-furanedione | - |
Mucor ambiguus | |
4.17 | - |
dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione | - |
Mucor ambiguus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
2 * 27500, SDS-PAGE | Mucor ambiguus |
44000 | - |
high-performance gel-permeation liquid chromatography, TSK G-3000 SW | Mucor ambiguus |
54000 | - |
gel filtration, Sephadex G-100 | Mucor ambiguus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mucor ambiguus | - |
- |
- |
Mucor ambiguus AKU 3006 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification to apparent homogenity | Mucor ambiguus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methylisatin + NADPH | - |
Mucor ambiguus | NADP+ + ? | - |
ir | |
5-bromoisatin + NADPH | - |
Mucor ambiguus | NADP+ + ? | - |
ir | |
5-methylisatin + NADPH | - |
Mucor ambiguus | NADP+ + ? | - |
ir | |
acenaphthenequinone + NADPH | reduced velocity | Mucor ambiguus | NADP+ + acenaphthylene-1,2-diol | - |
ir | |
alloxan + NADPH | - |
Mucor ambiguus | NADP+ + ? | - |
ir | |
alpha-naphthoquinone + NADPH | - |
Mucor ambiguus | NADP+ + naphthalene-1,4-diol | - |
ir | |
alpha-naphthoquinone + NADPH | - |
Mucor ambiguus AKU 3006 | NADP+ + naphthalene-1,4-diol | - |
ir | |
beta-naphthoquinone + NADPH | - |
Mucor ambiguus | NADP+ + naphthalene-1,2-diol | - |
ir | |
beta-naphthoquinone + NADPH | - |
Mucor ambiguus AKU 3006 | NADP+ + naphthalene-1,2-diol | - |
ir | |
dihydro-4-methyl-4-propyl-2,3-furanedione + NADPH | - |
Mucor ambiguus | NADP+ + 4-methyl-4-propyl-4,5-dihydrofuran-2,3-diol | - |
ir | |
dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione + NADPH | reduced velocity | Mucor ambiguus | NADP+ + 5-sec-butyl-4-ethyl-4-methyl-4,5-dihydrofuran-2,3-diol | - |
ir | |
dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH | - |
Mucor ambiguus | NADP+ + 4,4-dimethyl-5-(1-methylbutyl)-4,5-dihydrofuran-2,3-diol | - |
ir | |
dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione + NADPH | reduced velocity | Mucor ambiguus | NADP+ + 4-methyl-5-(1-methylbutyl)-4-propyl-4,5-dihydrofuran-2,3-diol | - |
ir | |
dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH | - |
Mucor ambiguus | NADP+ + 5-(1-ethylpropyl)-4,4-dimethyl-4,5-dihydrofuran-2,3-diol | - |
ir | |
dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione + NADPH | - |
Mucor ambiguus | NADP+ + 5-isopropyl-4,4-dimethyl-4,5-dihydrofuran-2,3-diol | - |
ir | |
isatin + NADPH | - |
Mucor ambiguus | NADP+ + 1-methyl-1H-indole-2,3-diol | - |
ir | |
ketopantoyl lactone + NADPH | - |
Mucor ambiguus | NADP+ + ? | - |
ir | |
ninhydrin + NADPH | - |
Mucor ambiguus | NADP+ + ? | - |
ir | |
p-benzoquinone + NADPH | - |
Mucor ambiguus | NADP+ + hydroquinone | - |
ir | |
p-benzoquinone + NADPH | - |
Mucor ambiguus AKU 3006 | NADP+ + hydroquinone | - |
ir | |
p-toluquinone + NADPH + H+ | - |
Mucor ambiguus | NADP+ + 2-methylbenzene-1,4-diol | - |
ir | |
p-toluquinone + NADPH + H+ | - |
Mucor ambiguus AKU 3006 | NADP+ + 2-methylbenzene-1,4-diol | - |
ir | |
phenanthrenequinone + NADPH | - |
Mucor ambiguus | NADP+ + ? | - |
ir | |
phenanthrenequinone + NADPH | - |
Mucor ambiguus AKU 3006 | NADP+ + ? | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27500, SDS-PAGE | Mucor ambiguus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
reduction of ketopantoyl lactone | Mucor ambiguus |
45 | - |
reduction of isatin | Mucor ambiguus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | for isatin and ketopantoyl lactone as substrates | Mucor ambiguus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | 15% of control activity at 50°C | Mucor ambiguus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | for isatin reduction | Mucor ambiguus |
6 | - |
for ketopantoyl lactone reduction | Mucor ambiguus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | with different buffers | Mucor ambiguus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | 91% of control activity at pH 9, 79% of control activity at pH 12, no activity at pH 4.0 | Mucor ambiguus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mucor ambiguus |