Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
oxidized 2,6-dichlorophenolindophenol | pH 6.5, 50°C | Aeropyrum pernix | |
0.28 | - |
L-proline | pH 6.5, 50°C | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, 68% loss of activity | Aeropyrum pernix | |
MgCl2 | 1 mM, 50% loss of activity | Aeropyrum pernix | |
additional information | no significant effect on activity with 1 mM SrCl2, CaCl2, CoCl2, MgCl2, CuCl2, ZnCl2 | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, SDS-PAGE | Aeropyrum pernix |
88000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.57 | - |
pH 6.5, 50°C | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-hydroxyproline + oxidized 2,6-dichlorophenolindophenol + H2O | reaction rate is 39% of that with L-proline | Aeropyrum pernix | ? | - |
? | |
L-proline + oxidized 2,6-dichlorophenolindophenol + H2O | the enzyme does not utilize ferricyanide or 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide as the electron acceptor, activity with phenazine methosulfate-p-iodonitrotetrazolium violet is 10% of that with 2,6-dichlorophenolindophenol | Aeropyrum pernix | (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | no activity with: D-proline, glycine, L-glutamate, L-aspartate, L-arginine, L-lysine, L-serine, L-leucine, L-alanine, L-threonine, L-methionine,L-tryptophan, L-tyrosine | Aeropyrum pernix | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47000, SDS-PAGE | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
dye-linked L-proline dehydrogenase | - |
Aeropyrum pernix |
LPDH | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Aeropyrum pernix |
75 | - |
above | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
10 min, stable | Aeropyrum pernix |
100 | - |
10 min, 48% loss of activity | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Aeropyrum pernix |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 12 | 50°C, 30 min, stable | Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Aeropyrum pernix |