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Literature summary for 1.5.3.20 extracted from

  • Gomi, K.; Horiuchi, T.
    Purification and characterization of a new enzyme, N-alkylglycine oxidase from Cladosporium sp. G-10 (1999), Biochim. Biophys. Acta, 1429, 439-445 .
    View publication on PubMed
Search for more literature for 1.5.3.20

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 2 mM, 53% residual activity Cladosporium sp.
iodoacetic acid 2 mM, 28% residual activity Cladosporium sp.
p-chloromercuribenzoic acid 0.2 mM, 38% residual activity Cladosporium sp.
sodium dodecylsulfate 2 mM, 34% residual activity Cladosporium sp.
ZnSO4 2 mM, 30% residual activity Cladosporium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 N-ethylglycine pH 8.0, 30°C Cladosporium sp.
0.97
-
 sarcosine pH 8.0, 30°C Cladosporium sp.
2.1
-
 Nepsilon-carboxymethyllysine pH 8.0, 30°C Cladosporium sp.
2.4
-
 N-carboxymethyl-6-aminocaproic acid pH 8.0, 30°C Cladosporium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Cladosporium sp.

Organism

Organism UniProt Comment Textmining
Cladosporium sp.
-
-
-
Cladosporium sp. G-10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cladosporium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2
-
 pH 8.0, 30°C Cladosporium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alkylglycine + H2O + O2
-
 Cladosporium sp. alkylamine + glyoxalate + H2O2
-
 ?
N-alkylglycine + H2O + O2
-
 Cladosporium sp. G-10 alkylamine + glyoxalate + H2O2
-
 ?
N-carboxymethyl-4-aminobutanoate + H2O + O2 12% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. glycine + 4-oxobutanoate + H2O2
-
 ?
N-carboxymethyl-4-aminobutanoate + H2O + O2 12% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. G-10 glycine + 4-oxobutanoate + H2O2
-
 ?
N-carboxymethyl-6-aminocaproic acid + H2O + O2 136% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. glycine + 6-oxo-caproic acid + H2O2
-
 ?
N-carboxymethyl-6-aminocaproic acid + H2O + O2 136% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. G-10 glycine + 6-oxo-caproic acid + H2O2
-
 ?
N-ethylglycine + H2O + O2 159% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. ethylamine + glyoxalate + H2O2
-
 ?
N-ethylglycine + H2O + O2 159% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. G-10 ethylamine + glyoxalate + H2O2
-
 ?
N-methylalanine + H2O + O2 42% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. methylamine + 2-oxo-propanoic acid + H2O2
-
 ?
N-methylalanine + H2O + O2 42% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. G-10 methylamine + 2-oxo-propanoic acid + H2O2
-
 ?
Nepsilon-carboxymethyllysine + H2O + O2
-
 Cladosporium sp. glycine + 6-oxo-lysine + H2O2
-
 ?
sarcosine + H2O + O2 135% of the rate with Nepsilon-carboxymethyllysine Cladosporium sp. methylamine + glyoxalate + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Cladosporium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Cladosporium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 stable below Cladosporium sp.
50
-
 10 min, complete loss of activity Cladosporium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10
-
 Cladosporium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9.5 stable for at least 10 min at 30°C Cladosporium sp.