Cloned (Comment) | Organism |
---|---|
the NADH-dependent flavin reductase is encoded by two highly similar genes, LJ_0548 and LJ_0549, or ,nfr1 and nfr2, encoding the subunits 1 and 2 of the enzyme, DNA and amino acid sequence determination and analysis, a plasmid with the promoter region of the LJ_0045 lactate dehydrogenase gene and a bidirectional terminator is used for overexpression of the LJ_0548 and LJ_0549 genes | Lactobacillus johnsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
FMN | pH 7.0, 37°C | Lactobacillus johnsonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | Lactobacillus johnsonii | - |
FADH2 + NAD+ | - |
r | |
FAD + NADH + H+ | Lactobacillus johnsonii NCC 533 | - |
FADH2 + NAD+ | - |
r | |
FMN + NADH + H+ | Lactobacillus johnsonii | - |
FMNH2 + NAD+ | - |
r | |
FMN + NADH + H+ | Lactobacillus johnsonii NCC 533 | - |
FMNH2 + NAD+ | - |
r | |
additional information | Lactobacillus acidophilus | no activity with NADPH | ? | - |
? | |
additional information | Lactobacillus acidophilus NCC 533 | no activity with NADPH | ? | - |
? | |
NADH + flavin | Lactobacillus acidophilus | - |
NAD+ + reduced flavin | - |
? | |
NADH + flavin | Lactobacillus acidophilus NCC 533 | - |
NAD+ + reduced flavin | - |
? | |
riboflavin + NADH + H+ | Lactobacillus johnsonii | - |
reduced riboflavin + NAD+ | - |
r | |
riboflavin + NADH + H+ | Lactobacillus johnsonii NCC 533 | - |
reduced riboflavin + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus acidophilus | Q74HL7 | subunit 1 | - |
Lactobacillus acidophilus | Q74HL8 | subunit 2 | - |
Lactobacillus acidophilus NCC 533 | Q74HL7 | subunit 1 | - |
Lactobacillus acidophilus NCC 533 | Q74HL8 | subunit 2 | - |
Lactobacillus johnsonii | Q74HL7 AND Q74HL8 | genes LJ_0548 and LJ_0549, or nfr1 and nfr2, encoding the subunits 1 and 2 of the enzyme | - |
Lactobacillus johnsonii NCC 533 | Q74HL7 AND Q74HL8 | genes LJ_0548 and LJ_0549, or nfr1 and nfr2, encoding the subunits 1 and 2 of the enzyme | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HiTrap Q column chromatography, and Superdex 200 gel filtration | Lactobacillus acidophilus |
native enzyme from strain NCC 533 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Lactobacillus johnsonii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
crude cell extract, about, with FAD, or FMN, or riboflavin, and NADH, pH 7.0, 37°C | Lactobacillus johnsonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | - |
Lactobacillus johnsonii | FADH2 + NAD+ | - |
r | |
FAD + NADH + H+ | - |
Lactobacillus johnsonii NCC 533 | FADH2 + NAD+ | - |
r | |
FMN + NADH + H+ | - |
Lactobacillus johnsonii | FMNH2 + NAD+ | - |
r | |
FMN + NADH + H+ | - |
Lactobacillus johnsonii NCC 533 | FMNH2 + NAD+ | - |
r | |
additional information | no activity with NADPH | Lactobacillus acidophilus | ? | - |
? | |
additional information | the reductase activity requires FMN, flavin adenine dinucleotide (FAD), or riboflavin, that give a similar activity, and is specific for NADH and not NADPH | Lactobacillus johnsonii | ? | - |
? | |
additional information | no activity with NADPH | Lactobacillus acidophilus NCC 533 | ? | - |
? | |
additional information | the reductase activity requires FMN, flavin adenine dinucleotide (FAD), or riboflavin, that give a similar activity, and is specific for NADH and not NADPH | Lactobacillus johnsonii NCC 533 | ? | - |
? | |
NADH + flavin | - |
Lactobacillus acidophilus | NAD+ + reduced flavin | - |
? | |
NADH + flavin | - |
Lactobacillus acidophilus NCC 533 | NAD+ + reduced flavin | - |
? | |
riboflavin + NADH + H+ | - |
Lactobacillus johnsonii | reduced riboflavin + NAD+ | - |
r | |
riboflavin + NADH + H+ | - |
Lactobacillus johnsonii NCC 533 | reduced riboflavin + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, SDS-PAGE | Lactobacillus acidophilus |
dimer | 2 * 20000, SDS-PAGE | Lactobacillus johnsonii |
More | the two 20-kDa subunit proteins contain flavin mononucleotide (FMN) reductase conserved domains | Lactobacillus johnsonii |
Synonyms | Comment | Organism |
---|---|---|
flavin mononucleotide reductase | - |
Lactobacillus johnsonii |
FMN reductase | - |
Lactobacillus johnsonii |
LJ0548 | - |
Lactobacillus acidophilus |
LJ0549 | - |
Lactobacillus acidophilus |
LJ_0548 | - |
Lactobacillus johnsonii |
LJ_0549 | - |
Lactobacillus johnsonii |
NADH-dependent flavin reductase | - |
Lactobacillus johnsonii |
NADH-dependent flavin reductase | - |
Lactobacillus acidophilus |
nfr1 | - |
Lactobacillus johnsonii |
nfr2 | - |
Lactobacillus johnsonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactobacillus johnsonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Lactobacillus johnsonii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Lactobacillus acidophilus | |
FMN | - |
Lactobacillus acidophilus | |
NADH | - |
Lactobacillus acidophilus | |
riboflavin | - |
Lactobacillus acidophilus |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the encoding genes genes nfr1 and nfr2 in Lactobacillus johnsonii leads to a 40fold reduction of hydrogen peroxide formation. H2O2 production in this mutant can only be restored by in trans complementation of both genes | Lactobacillus johnsonii |
physiological function | the conserved NADH-dependent flavin reductase is prominently involved in H2O2 production in Lactobacillus johnsonii, overview | Lactobacillus johnsonii |