Application | Comment | Organism |
---|---|---|
synthesis | folate production by the mutant L62F in two-stage fermentation process with temperature shift-up from 30°C to 40°C increases by three-fold compared with the parental strain | Lactiplantibacillus plantarum |
Protein Variants | Comment | Organism |
---|---|---|
L62F | thermosensitive, mutant strain is to grow at 30°C but not at 40°C. Mutant protein shows a reduction in DHFR activity from 1.51 to 0.06 Units/mg protein, resulting in increasing DHF amount at the expense of THF. Total folate production by the mutant in two-stage fermentation process with temperature shift-up from 30°C to 40°C increases by three-fold compared with the parental strain | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | A0A0S4L1K0 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
mutant L62F, pH not specified in the publication, temperature not specified in the publication | Lactiplantibacillus plantarum |
1.51 | - |
wild-type, pH not specified in the publication, temperature not specified in the publication | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Lactiplantibacillus plantarum | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DfrA | - |
Lactiplantibacillus plantarum |