Literature summary for 1.5.1.3 extracted from

Thapliyal, C.; Jain, N.; Rashid, N.; Chaudhuri, P.
Kinetics and thermodynamics of the thermal inactivation and chaperone assisted folding of zebrafish dihydrofolate reductase (2018), Arch. Biochem. Biophys., 637, 21-30 .

Search for more literature for 1.5.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Danio rerio

Organism

Organism	UniProt	Comment	Textmining
Danio rerio	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
both the chaperone systems GroEL (minichaperone) and GroEL-GroES prevent thermal aggregation and assist in refolding of DHFR	Danio rerio

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
47	-	half-life 5.18 min, in presence of GroEL (minichaperone) half-life 12.35 min, in presence of GroEL-GroES half-life 21.32 min	Danio rerio
50	-	complete unfolding and loss of activity. The heat induced inactivation follows first-order kinetics and Arrhenius law	Danio rerio
50	-	half-life 2.13 min, in presence of GroEL (minichaperone) half-life 8.09 min, in presence of GroEL-GroES half-life 16.54 min	Danio rerio
55	-	half-life 1.33 min, in presence of GroEL (minichaperone) half-life 3.44 min, in presence of GroEL-GroES half-life 7.19 min	Danio rerio

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)