Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Lacticaseibacillus casei |
comparison of the backbone conformation in crystal structures, detection of mutational insertions | Bacteria |
comparison of the backbone conformation in crystal structures, detection of mutational insertions | vertebrata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,6-Trinitrobenzenesulfonic acid | - |
Enterococcus faecium | |
2,4-Diamino-5-adamantylpyrimidine | weak inhibition | Gallus gallus | |
2,4-Diamino-5-adamantylpyrimidine | weak inhibition | vertebrata | |
2,4-Diamino-5-benzylpyrimidines | - |
Gallus gallus | |
2,4-Diamino-5-phenyl-s-triazines | - |
Gallus gallus | |
2,4-Pentanedione | - |
Lacticaseibacillus casei | |
4,6-Diamino-1,2-dihydro-2,2-dimethyl-1-(phenylalkylphenyl)-s-triazines | bearing a terminal sulfonyl fluoride | Gallus gallus | |
Dansyl chloride | - |
Lacticaseibacillus casei | |
diethyldicarbonate | - |
Enterococcus faecium | |
diethyldicarbonate | - |
Escherichia coli | |
diethyldicarbonate | - |
Lacticaseibacillus casei | |
diethyldicarbonate | - |
Mus musculus | |
methotrexate | dihydrofolate antagonist drug | Bacteria | |
methotrexate | - |
Bos taurus | |
methotrexate | - |
Crithidia fasciculata | |
methotrexate | dihydrofolate antagonist drug | Escherichia coli | |
methotrexate | also ornithine, lysine and dansyl analogs of methotrexate | Gallus gallus | |
methotrexate | dihydrofolate antagonist drug | Homo sapiens | |
methotrexate | - |
Lacticaseibacillus casei | |
methotrexate | dihydrofolate antagonist drug | Mammalia | |
methotrexate | dihydrofolate antagonist drug | Mus musculus | |
methotrexate | - |
Sus scrofa | |
methotrexate | - |
Tequatrovirus T4 | |
additional information | stereochemistry of inhibitor binding | Bacteria | |
additional information | - |
Bos taurus | |
additional information | - |
Crithidia fasciculata | |
additional information | - |
Enterococcus faecium | |
additional information | stereochemistry of inhibitor binding | Escherichia coli | |
additional information | stereochemistry of inhibitor binding | Gallus gallus | |
additional information | stereochemistry of inhibitor binding | Lacticaseibacillus casei | |
additional information | - |
Mus musculus | |
additional information | stereochemistry of inhibitor binding | protozoa | |
additional information | - |
Sus scrofa | |
additional information | stereochemistry of inhibitor binding | vertebrata | |
N-bromosuccinimide | - |
Lacticaseibacillus casei | |
organic mercurials | animal enzyme: activated, bacterial enzyme: unaffected or inhibited | Enterococcus faecium | |
organic mercurials | animal enzyme: activated, bacterial enzyme: unaffected or inhibited | Escherichia coli | |
Phenylglyoxal | total inactivation | Lacticaseibacillus casei | |
trimethoprim | folate antagonist drug | Bacteria | |
trimethoprim | - |
Bos taurus | |
trimethoprim | - |
Crithidia fasciculata | |
trimethoprim | - |
Escherichia coli | |
trimethoprim | - |
Gallus gallus | |
trimethoprim | - |
Homo sapiens | |
trimethoprim | - |
Lacticaseibacillus casei | |
trimethoprim | folate antagonist drug | Mammalia | |
trimethoprim | folate antagonist drug | Mus musculus | |
trimethoprim | - |
Sus scrofa | |
trimethoprim | - |
vertebrata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mammalia | |
additional information | - |
additional information | - |
Tequatrovirus T4 | |
additional information | - |
additional information | overview | Gallus gallus | |
additional information | - |
additional information | overview | Bacteria | |
additional information | - |
additional information | overview | Mus musculus | |
additional information | - |
additional information | overview | Escherichia coli | |
additional information | - |
additional information | overview | Homo sapiens | |
additional information | - |
additional information | overview | Sus scrofa | |
additional information | - |
additional information | overview | Bos taurus | |
additional information | - |
additional information | overview | Crithidia fasciculata | |
additional information | - |
additional information | overview | Lacticaseibacillus casei | |
additional information | - |
additional information | overview | Enterococcus faecium | |
additional information | - |
additional information | overview | vertebrata | |
additional information | - |
additional information | overview | protozoa | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Gallus gallus | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Bacteria | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Mus musculus | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Escherichia coli | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Homo sapiens | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Sus scrofa | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Bos taurus | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Crithidia fasciculata | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Lacticaseibacillus casei | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | Enterococcus faecium | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | vertebrata | |
additional information | - |
additional information | Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes | protozoa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
organic mercurials | - |
Mammalia | |
organic mercurials | - |
Lacticaseibacillus casei | |
organic mercurials | animal enzyme: activated, bacterial enzyme: unaffected or inhibited | Gallus gallus | |
organic mercurials | animal enzyme: activated, bacterial enzyme: unaffected or inhibited | Mus musculus | |
organic mercurials | animal enzyme: activated, bacterial enzyme: unaffected or inhibited | Homo sapiens | |
organic mercurials | animal enzyme: activated, bacterial enzyme: unaffected or inhibited | Sus scrofa | |
organic mercurials | animal enzyme: activated, bacterial enzyme: unaffected or inhibited | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18250 | - |
MB 1428, amino acid sequence | Escherichia coli |
18300 | - |
amino acid sequence | Lacticaseibacillus casei |
19600 | - |
isoenzyme II, amino acid sequence | Enterococcus faecium |
21450 | - |
amino acid sequence | Sus scrofa |
21450 | - |
amino acid sequence | Bos taurus |
21460 | - |
methotrexate resistant L1210(R) cells, amino acid sequence | Mus musculus |
21650 | - |
amino acid sequence | Gallus gallus |
56700 | - |
2 * 56700, bifunctional enzyme: thymidylate synthase-dihydrofolate reductase, SDS-PAGE | Crithidia fasciculata |
107000 | - |
bifunctional enzyme: thymidylate synthase-dihydrofolate reductase | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteria | - |
overview | - |
Bos taurus | - |
- |
- |
Crithidia fasciculata | - |
bifunctional enzyme: dihydrofolate reductase-thymidylate synthase EC 2.1.1.45 | - |
Enterococcus faecium | - |
- |
- |
Escherichia coli | - |
various strains | - |
Gallus gallus | - |
- |
- |
Homo sapiens | - |
methotrexate-resistant WIL-2 lymphoblastoid cells | - |
Lacticaseibacillus casei | - |
- |
- |
Mammalia | - |
cultured mammalian cells, overview | - |
Mus musculus | - |
L1210 lymphoma cells, amethopterin-resistant | - |
protozoa | - |
overview | - |
Streptococcus pneumoniae | - |
methotrexate-resistant form | - |
Sus scrofa | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
vertebrata | - |
overview | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | catalytic mechanism | Bacteria | |
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | catalytic mechanism | Escherichia coli | |
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | catalytic mechanism | Lacticaseibacillus casei | |
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | catalytic mechanism | Enterococcus faecium | |
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | catalytic mechanism | vertebrata | |
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | catalytic mechanism | protozoa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
liver | - |
Mus musculus | - |
liver | - |
Sus scrofa | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
methotrexate resistant cells, L1210 (R), 25°C | Mus musculus |
12 | - |
30°C | Lacticaseibacillus casei |
14 | - |
chicken liver, 28°C | Gallus gallus |
15 | - |
human WIL2 (M4) cells, 22°C | Homo sapiens |
26 | - |
bovine liver, 28°C | Bos taurus |
27 | - |
methotrexate resistant cells, sarcoma 180 (AT/3000), 37°C | Mammalia |
36 | - |
methotrexate resistant, 23°C | Escherichia coli |
36 | - |
liver, 30°C | Sus scrofa |
45 | - |
- |
Escherichia coli |
45 | - |
methotrexate resistant cells, isoenzyme II, 37°C | Enterococcus faecium |
55 | - |
trimethoprim resistant, 30°C | Escherichia coli |
60 | - |
human KB cells, 37°C | Homo sapiens |
300 | - |
isoenzyme I | Enterococcus faecium |
300 | - |
25°C | Enterococcus faecium |
300 | - |
methotrexate resistant cells | Enterococcus faecium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Gallus gallus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Mammalia | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Bacteria | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Mus musculus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Sus scrofa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Lacticaseibacillus casei | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Enterococcus faecium | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
vertebrata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
protozoa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Bacteria |
dimer | - |
Escherichia coli |
dimer | 2 * 56700, bifunctional enzyme: thymidylate synthase-dihydrofolate reductase, SDS-PAGE | Crithidia fasciculata |
monomer | - |
Bacteria |
monomer | - |
Tequatrovirus T4 |
monomer | 1 * 21650, amino acid sequence | Gallus gallus |
monomer | 1 * 21450, amino acid sequence | Sus scrofa |
monomer | 1 * 21450, amino acid sequence | Bos taurus |
monomer | 1 * 21460, methotrexate resistant L1210 (R) cells, amino acid sequence | Mus musculus |
monomer | 1 * 18250, MB 1428, amino acid sequence | Escherichia coli |
monomer | 1 * 19600, isoenzyme II, amino acid sequence | Enterococcus faecium |
monomer | 1 * 18300, amino acid sequence | Lacticaseibacillus casei |
More | - |
Crithidia fasciculata |
More | overview | Bacteria |
More | overview | Tequatrovirus T4 |
More | overview | protozoa |
tetramer | 4 * 8500-9000, R-plasmid-coded type II reductase | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.67 | - |
NADPH | pH 6.5 | Lacticaseibacillus casei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
methotrexate resistant cells L1210 (R), sarcoma 180 (AT/300), 2 optima: pH 4 and pH 7.5 | Mammalia |
4 | - |
methotrexate resistant cells L1210 (R), sarcoma 180 (AT/300), 2 optima: pH 4 and pH 7.5 | Mus musculus |
4 | - |
7,8-dihydrofolate + NADPH, 2 optima: pH 4 and pH 7.4 | Gallus gallus |
4 | - |
human WIL 2 cell line, 2 optima: pH 4 and pH 7.3-8.3 | Homo sapiens |
4.8 | 5 | - |
Bos taurus |
5 | - |
2 optima: pH 5 and pH 7.6 | Sus scrofa |
5.8 | - |
7,8-dihydrofolate, isoenzyme II, methotrexate resistant cells | Enterococcus faecium |
6.5 | - |
- |
Escherichia coli |
6.5 | - |
- |
Lacticaseibacillus casei |
6.5 | - |
isoenzyme I, methotrexate resistant cells | Enterococcus faecium |
6.8 | - |
- |
Crithidia fasciculata |
6.8 | - |
MB 1328 | Escherichia coli |
7.2 | 8.2 | KB cells, 2 optima: below pH 5.0 and pH 7.2-8.2 | Homo sapiens |
7.3 | 8.3 | WIL2 (M4) cells, 2 optima: pH 4 and pH 7.3-8.3 | Homo sapiens |
7.4 | - |
7,8-dihydrofolate + NADPH, 2 optima: pH 4.0 and pH 7.4 | Gallus gallus |
7.5 | - |
- |
Homo sapiens |
7.5 | - |
methotrexate resistant cells, L1210(R), sarcoma 180 (AT/30000), 2 optima: pH 4.0 and pH 7.5 | Mammalia |
7.5 | - |
methotrexate resistant cells, L1210(R), sarcoma 180 (AT/30000), 2 optima: pH 4.0 and pH 7.5 | Mus musculus |
7.6 | - |
- |
Escherichia coli |
7.6 | - |
- |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Gallus gallus | |
NADPH | - |
Mammalia | |
NADPH | - |
Mus musculus | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Sus scrofa | |
NADPH | - |
Bos taurus | |
NADPH | - |
Crithidia fasciculata | |
NADPH | - |
Streptococcus pneumoniae | |
NADPH | - |
Lacticaseibacillus casei | |
NADPH | - |
Enterococcus faecium | |
NADPH | - |
Tequatrovirus T4 | |
NADPH | interaction study | Bacteria | |
NADPH | interaction study | vertebrata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Gallus gallus | |
additional information | - |
additional information | - |
Mus musculus | |
additional information | - |
additional information | - |
Escherichia coli | |
additional information | - |
additional information | - |
Homo sapiens | |
additional information | - |
additional information | - |
Lacticaseibacillus casei | |
additional information | - |
additional information | overview, effect of methotrexate and trimethoprim | Bacteria | |
additional information | - |
additional information | overview, effect of methotrexate and trimethoprim | vertebrata | |
additional information | - |
additional information | overview, effect of methotrexate and trimethoprim | protozoa |