Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.3 extracted from

  • Ahn, M.; Park, S.; Jeon, J.; Choi, J.K.; Khang, Y.
    Application of an S-layer protein as a self-aggregating tag for cost-effective separation of recombinant human and yeast D-amino acid oxidases in the aqueous two-phase system (2020), Biotechnol. Lett., 42, 241-248 .
    View publication on PubMed
Search for more literature for 1.4.3.3

Application

Application Comment Organism
synthesis use of surface layer (S-layer) protein of Lactobacillus brevis as a self-aggregating protein tag to enable cost-effective separation of human and yeast D-amino acid oxidases expressed in Escherichia coli cells. Human and yeast D-amino acid oxidases fused with S-layer proteins can be easily separated by aggregates at the interface and/or in a few conditions by precipitates to the bottom of the PEG-phosphate aqueous system Rhodotorula toruloides
synthesis use of surface layer (S-layer) protein of Lactobacillus brevis as a self-aggregating protein tag to enable cost-effective separation of human and yeast D-amino acid oxidases expressed in Escherichia coli cells. Human and yeast D-amino acid oxidases fused with S-layer proteins can be easily separated by aggregates at the interface and/or in a few conditions by precipitates to the bottom of the PEG-phosphate aqueous system Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodotorula toruloides
expression in Escherichia coli Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P14920
-
-
Rhodotorula toruloides P80324
-
-
Rhodotorula toruloides ATCC 26217 P80324
-
-

Purification (Commentary)

Purification (Comment) Organism
use of surface layer (S-layer) protein of Lactobacillus brevis as a self-aggregating protein tag to enable cost-effective separation of human and yeast D-amino acid oxidases expressed in Escherichia coli cells. Human and yeast D-amino acid oxidases fused with S-layer proteins can be easily separated by aggregates at the interface and/or in a few conditions by precipitates to the bottom of the PEG-phosphate aqueous system Rhodotorula toruloides
use of surface layer (S-layer) protein of Lactobacillus brevis as a self-aggregating protein tag to enable cost-effective separation of human and yeast D-amino acid oxidases expressed in Escherichia coli cells. Human and yeast D-amino acid oxidases fused with S-layer proteins can be easily separated by aggregates at the interface and/or in a few conditions by precipitates to the bottom of the PEG-phosphate aqueous system Homo sapiens