Literature summary for 1.4.3.3 extracted from

Yang, S.
Efficient production of D-amino acid oxidase in Escherichia coli by a trade-off between its expression and biomass using N-terminal modification (2017), Biores. Technol., 243, 716-723 .

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Application

Application	Comment	Organism
synthesis	the soluble expression of is improved in Escherichia coli through N-terminal modification, but the cell biomass is decreased. When a variant carrying an additional glycine in position 3 is fused with three N-terminus histidine residues, the volumetric activity is increased by 3.1 times and the biomass is not significant change compared with the wild type. When the N-terminal disordered region of DAAO (HSQK) is replaced with HHHG, the variant enzyme activity reaches 80.7 U/ml in a 7.5 l fermenter in 24 h	Rhodotorula toruloides

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Rhodotorula toruloides	P80324	-	-

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Release 2023.1 (January 2023)