Literature summary for 1.4.3.3 extracted from

Murtas, G.; Caldinelli, L.; Cappelletti, P.; Sacchi, S.; Pollegioni, L.
Human D-amino acid oxidase The inactive G183R variant (2018), Biochim. Biophys. Acta, 1866, 822-830 .

Search for more literature for 1.4.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and in U87 cell	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
G183R	inactive apoprotein, substitution negatively affects the ability to bind the flavin cofactor in the correct orientation. The overexpressed G183R protein is not fully targeted to peroxisomes, shows colocalization with ubiquitin, and increases 7fold both the D-serine cellular concentration and the D/(D+L)-serine ratio	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	gel filtration, both wild-type and mutant G183R	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P14920	-	-

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
53.1	-	wild-type, melting temperature, via protein fluorescence	Homo sapiens
58.8	-	wild-type, melting temperature, via circular dichroism	Homo sapiens
59.1	-	mutant G183R, melting temperature, via protein fluorescence	Homo sapiens
63.2	-	mutant G183R, melting temperature, via circular dichroism	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)