Cloned (Comment) | Organism |
---|---|
the His-tagged enzyme is expressed in Escherichia coli | Trigonopsis variabilis |
General Stability | Organism |
---|---|
His-tagged DAAO loses its flavin cofactor upon dilution or prolonged dialysis against FAD-free solvents leading to the catalytic inactivation | Trigonopsis variabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
D-alanine | apparent value, reconstituted holoenzyme at pH 8.0 and 30°C | Trigonopsis variabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trigonopsis variabilis | Q99042 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Trigonopsis variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Trigonopsis variabilis | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Trigonopsis variabilis | pyruvate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Trigonopsis variabilis |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Trigonopsis variabilis |
DAAO | - |
Trigonopsis variabilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | loss of secondary structure and inactivation occurs over a range of 30°C to 45°C, the melting temperature is at 41.4°C | Trigonopsis variabilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
95 | - |
D-alanine | apparent value, reconstituted holoenzyme at pH 8.0 and 30°C | Trigonopsis variabilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains non-covalently bound FAD | Trigonopsis variabilis |