Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Bothrops pictus | |
glutathione | - |
Bothrops pictus | |
iodoacetate | - |
Bothrops pictus | |
additional information | not inhibitory: Ca2+, Mg2+ and Mn2+, PMSF, EDTA and glutamic acid | Bothrops pictus | |
Zn2+ | - |
Bothrops pictus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
132000 | - |
gel filtration | Bothrops pictus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops pictus | X2L4E2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bothrops pictus |
proteolytic modification | sequence contains a signal peptide of 11 amino acids | Bothrops pictus |
Purification (Comment) | Organism |
---|---|
by two chromatographic steps on Sephadex G-100 and CM Sephadex C-50 | Bothrops pictus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops pictus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + H2O + O2 | - |
Bothrops pictus | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 65000, SDS-PAGE | Bothrops pictus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | - |
Bothrops pictus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
rapid loss of activity above | Bothrops pictus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
stable up to | Bothrops pictus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bothrops pictus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Bothrops pictus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bothrops pictus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is evolutively close related to LAAOs from Bothrops jararacussu, Bothrops moojeni and Bothrops atrox, and together with the LAAO from Bothrops pauloensis, they form a well-defined cluster of the Bothrops genus | Bothrops pictus |
additional information | the enzyme induces edema in mice, inhibits human platelet aggregation induced by ADP in a dose-dependent manner and shows antibacterial activity on Gram-positive and Gram-negative bacteria | Bothrops pictus |