Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrurus lemniscatus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme contains a large proportion of typical N- (about 38.0%) and O- (about 23.0%) linked glycoconjugates | Micrurus lemniscatus |
Purification (Comment) | Organism |
---|---|
by size exclusion chromatography | Micrurus lemniscatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Micrurus lemniscatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
800 | - |
substrate L-tyrosine, pH 8.5, 37°C | Micrurus lemniscatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + H2O + O2 | - |
Micrurus lemniscatus | 2-oxo-4-methylpentanoate + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | - |
Micrurus lemniscatus | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
L-tyrosine + H2O + O2 | - |
Micrurus lemniscatus | 3-(4-hydroxyphenyl)pyruvate + NH3 + H2O2 | - |
? | |
additional information | enzyme shows high specificity for Tyr, Trp and Leu and moderate specificity for Phe > Met > Thr, while other amino acids as Ser, Gly, Lys and Pro are not oxidized | Micrurus lemniscatus | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, SDS-PAGE and MALDI-TOF | Micrurus lemniscatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Micrurus lemniscatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Micrurus lemniscatus | presence of several isoform, pI 6.3-8.9, isoelectric focusing | - |
additional information |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme blocks aggregation induced by collagen on washed blood platelets in a rather weak manner, it does not inhibit platelet aggregation induced by ADP on platelet-rich plasma. The enzyme displays in vitro antibacterial activity against Staphylococcus aureus (MIC/MBC of 0.39 microg/ml) and in vitro leishmanicidal action against Leishmania amazonensis and L. chagasi (IC50 values of 0.14 and 0.039 microg/ml, respectively). These activities are significantly reduced by catalase | Micrurus lemniscatus |