Cloned (Comment) | Organism |
---|---|
expression in Sf9 cell, the recombinant baculovirus rSR-LAAO is abundantly expressed in infected Sf9 insect cells | Siganus canaliculatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pepsin | 10 mg/ml, complete inhibition | Siganus canaliculatus | |
proteinase K | 10 mg/ml, complete inhibition | Siganus canaliculatus | |
Trypsin | 10 mg/ml, complete inhibition | Siganus canaliculatus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | treatment reduces the relative bacteriostatic activity to 62.35% | Siganus canaliculatus |
Ethanol | adding an equal volume of ethanol has a minimal impact on the antibacterial activity of crude recombinant LAAO | Siganus canaliculatus |
Methanol | treatment reduces the relative bacteriostatic activity to 62.54% | Siganus canaliculatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Siganus canaliculatus | P86810 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + H2O + O2 | - |
Siganus canaliculatus | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE, x * 55948, calculated from sequence | Siganus canaliculatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 30 | crude enzyme can maintain a high level of antibacterial activity against both Gram-positive and -negative bacteria from 4°C to 30°C | Siganus canaliculatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Siganus canaliculatus | calculated from sequence | - |
5.9 |
General Information | Comment | Organism |
---|---|---|
additional information | crude recombinant LAAO enzyme displays strong antibacterial activity against aquatic pathogens, particularly Streptococcus agalactiae and Streptococcus iniae. The antibacterial activity of recombinant LAAO is directly associated with H2O2 production, the crude enzyme can maintain a high level of antibacterial activity against both Gram-positive and -negative bacteria from 4°C to 30°C | Siganus canaliculatus |