Crystallization (Comment) | Organism |
---|---|
native isozyme LAAO I from venom, sitting drop vapour-diffusion method, mixing of 0.001 ml of protein solution with 10 mg/ml protein with 0.001 ml of a precipitant solution consisting of 16% w/v PEG 3350 and 0.04 M zinc acetate against 1 ml of reservoir solution at 20°C for 2 days, X-ray diffraction structure determination and analysis at 2.6 A resolution | Vipera ammodytes ammodytes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | venom | Vipera ammodytes ammodytes | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, LAAO I, SDS-PAGE | Vipera ammodytes ammodytes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an L-amino acid + H2O + O2 | Vipera ammodytes ammodytes | L-amino-acid oxidase catalyzes the oxidative deamination of a number of L-amino acids to their respective imino acids with concomitant hydrolysis to alpha-keto acids, together with the production of ammonia and hydrogen peroxide | a 2-oxo-acid + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vipera ammodytes ammodytes | - |
captured in northwest Bulgaria, five LAAO isozymes | - |
Purification (Comment) | Organism |
---|---|
native isozymes from venom by 2times anion exchange chromatography and dialysis to homogeneity | Vipera ammodytes ammodytes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | five LAAO isozymes | Vipera ammodytes ammodytes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an L-amino acid + H2O + O2 | L-amino-acid oxidase catalyzes the oxidative deamination of a number of L-amino acids to their respective imino acids with concomitant hydrolysis to alpha-keto acids, together with the production of ammonia and hydrogen peroxide | Vipera ammodytes ammodytes | a 2-oxo-acid + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, LAAO I, SDS-PAGE | Vipera ammodytes ammodytes |
More | peptide mapping by mass spectrometry | Vipera ammodytes ammodytes |
Synonyms | Comment | Organism |
---|---|---|
LAAO | - |
Vipera ammodytes ammodytes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | non-covalently bound | Vipera ammodytes ammodytes |