Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | inhibits the enzyme at physiological concentrations by competing with the flavin for binding to the apoprotein | Arabidopsis thaliana | |
NADP+ | inhibits the enzyme at physiological concentrations by competing with the flavin for binding to the apoprotein | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
L-aspartate | pH 8.0, 20°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
SDS-PAGE | Arabidopsis thaliana |
127000 | - |
native gel electrophoresis | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + fumarate | Arabidopsis thaliana | - |
oxaloacetate + NH3 + succinate | - |
? | |
L-aspartate + O2 | Arabidopsis thaliana | first enzyme of the de novo biosynthetic pathway of NAD+ in plants | iminosuccinate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + fumarate | - |
Arabidopsis thaliana | oxaloacetate + NH3 + succinate | - |
? | |
L-aspartate + O2 | - |
Arabidopsis thaliana | iminosuccinate + H2O2 | - |
? | |
L-aspartate + O2 | first enzyme of the de novo biosynthetic pathway of NAD+ in plants | Arabidopsis thaliana | iminosuccinate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
At5g14760 | - |
Arabidopsis thaliana |
LASPO | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | 40°C: about 55% of maximal activity, 65°C: about 40% of maximal activity | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
L-aspartate | pH 8.0, 20°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | pH 7.0: about 55% of maximal activity, pH 12.0: about 80% of maximal activity | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes a key metabolic step for the production of the nucleotide cofactor NAD+ | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
L-aspartate | pH 8.0, 20°C | Arabidopsis thaliana |