Application | Comment | Organism |
---|---|---|
environmental protection | as a potential biocatalyst, the enzyme is promising in controlling cyclohexylamine pollution and deracemization of chiral amines | Acinetobacter sp. YT-02 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) | Acinetobacter sp. YT-02 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 2 mM, 10% loss of activity | Acinetobacter sp. YT-02 | |
Co2+ | 2 mM, 15% loss of activity | Acinetobacter sp. YT-02 | |
K+ | 2 mM, 17% loss of activity | Acinetobacter sp. YT-02 | |
Mg2+ | 2 mM, 28% loss of activity | Acinetobacter sp. YT-02 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
Cyclohexylamine | pH 7.0, 30°C | Acinetobacter sp. YT-02 | |
0.25 | - |
Cyclohexylamine | pH 7.0, 50°C | Acinetobacter sp. YT-02 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
gel filtration | Acinetobacter sp. YT-02 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexylamine + O2 + H2O | Acinetobacter sp. YT-02 | - |
cyclohexanone + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. YT-02 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni affinity chromatography | Acinetobacter sp. YT-02 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylcyclohexanamine + O2 + H2O | 22% of the activity with cyclohexylamine | Acinetobacter sp. YT-02 | 2-methylcyclohexanone + NH3 + H2O2 | - |
? | |
4-methylcyclohexanamine + O2 + H2O | 41% of the activity with cyclohexylamine | Acinetobacter sp. YT-02 | 4-methylcyclohexanone + NH3 + H2O2 | - |
? | |
cycloheptylamine + O2 + H2O | 55% of the activity with cyclohexylamine | Acinetobacter sp. YT-02 | cycloheptanone + NH3 + H2O2 | - |
? | |
cyclohexylamine + O2 + H2O | - |
Acinetobacter sp. YT-02 | cyclohexanone + NH3 + H2O2 | - |
? | |
cyclopentylamine + O2 + H2O | 7% of the activity with cyclohexylamine | Acinetobacter sp. YT-02 | cyclopentanone + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Acinetobacter sp. YT-02 |
Synonyms | Comment | Organism |
---|---|---|
CF596_10820 | - |
Acinetobacter sp. YT-02 |
CHAO | - |
Acinetobacter sp. YT-02 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Acinetobacter sp. YT-02 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: about 70% of maximal activity, 60°C: about 70% of maximal activity | Acinetobacter sp. YT-02 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, pH 7.0, more than 40% activity of the enzyme is retained | Acinetobacter sp. YT-02 |
50 | - |
30 min, activity decreases rapidly when the temperature is higher than 50°C | Acinetobacter sp. YT-02 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
432 | - |
Cyclohexylamine | pH 7.0, 30°C | Acinetobacter sp. YT-02 | |
523 | - |
Cyclohexylamine | pH 7.0, 50°C | Acinetobacter sp. YT-02 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Acinetobacter sp. YT-02 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | the enzyme retains more than 60% residual activity at pH 7.0-9.0 | Acinetobacter sp. YT-02 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | enzyme activity is maintained at more than 50% level after incubation for 30 min at pH 7.0-9.0 | Acinetobacter sp. YT-02 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1724 | - |
Cyclohexylamine | pH 7.0, 30°C | Acinetobacter sp. YT-02 | |
2075 | - |
Cyclohexylamine | pH 7.0, 50°C | Acinetobacter sp. YT-02 |