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Literature summary for 1.4.3.12 extracted from

  • Zhang, J.D.; Chang, Y.W.; Dong, R.; Yang, X.X.; Gao, L.L.; Li, J.; Huang, S.P.; Guo, X.M.; Zhang, C.F.; Chang, H.H.
    Enantioselective cascade biocatalysis for deracemization of racemic beta-amino alcohols to enantiopure (S)-beta-amino alcohols by employing cyclohexylamine oxidase and omega-transaminase (2021), ChemBioChem, 22, 124-128 .
    View publication on PubMed
Search for more literature for 1.4.3.12

Application

Application Comment Organism
synthesis deracemization of racemic beta-amino alcohols is conducted by cyclohexylamine oxidase-catalyzed enantioselective deamination and transaminase-catalyzed enantioselective amination to afford (S)-beta-amino alcohols in excellent conversion (78-94%) and enantiomeric excess (above 99%) Arthrobacter sp. TYUT010-15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Arthrobacter sp. TYUT010-15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 (1R,2S)-1-amino-2-indanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
0.7
-
 (1S,2R)-1-amino-2-indanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
0.9
-
 (1S,2S)-2-aminocyclopentanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
1
-
 (1S,2S)-2-aminocyclohexanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
1.1
-
 (1R,2R)-2-aminocyclopentanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
1.3
-
 (1R,2R)-2-aminocyclohexanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. TYUT010-15 A0A7G7Y0T8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter sp. TYUT010-15

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,2R)-2-aminocyclohexanol + O2 + H2O the enzyme shows highly R-selective deamination activity toward beta-amino alcohols Arthrobacter sp. TYUT010-15 cyclohexanone + NH3 + H2O2
-
 ?
(1R,2R)-2-aminocyclopentanol + O2 + H2O the enzyme shows highly R-selective deamination activity toward beta-amino alcohols Arthrobacter sp. TYUT010-15 cyclopentanone + NH3 + H2O2
-
 ?
(1R,2S)-1-amino-2-indanol + O2 + H2O the enzyme shows highly R-selective deamination activity toward beta-amino alcohols Arthrobacter sp. TYUT010-15 ? + NH3 + H2O2
-
 ?
(1S,2R)-1-amino-2-indanol + O2 + H2O the enzyme shows highly R-selective deamination activity toward beta-amino alcohols Arthrobacter sp. TYUT010-15 ? + NH3 + H2O2
-
 ?
(1S,2S)-2-aminocyclohexanol + O2 + H2O the enzyme shows highly R-selective deamination activity toward beta-amino alcohols Arthrobacter sp. TYUT010-15 cyclohexanone + NH3 + H2O2
-
 ?
(1S,2S)-2-aminocyclopentanol + O2 + H2O the enzyme shows highly R-selective deamination activity toward beta-amino alcohols Arthrobacter sp. TYUT010-15 cyclopentanone + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
ArCHAO
-
 Arthrobacter sp. TYUT010-15

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Arthrobacter sp. TYUT010-15

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 70% of maximal activity, 50°C: about 60% of maximal activity Arthrobacter sp. TYUT010-15

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 30 pH 7.0, 24 h, 100 mM sodium phosphate buffer, more than 70% residual activity Arthrobacter sp. TYUT010-15
50
-
 pH 7.0, 24 h, 100 mM sodium phosphate buffer, 10% residual activity Arthrobacter sp. TYUT010-15

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 (1S,2R)-1-amino-2-indanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
1.5
-
 (1S,2S)-2-aminocyclohexanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
1.6
-
 (1S,2S)-2-aminocyclopentanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
4.9
-
 (1R,2S)-1-amino-2-indanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
5.6
-
 (1R,2R)-2-aminocyclopentanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
5.8
-
 (1R,2R)-2-aminocyclohexanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Arthrobacter sp. TYUT010-15

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 70% of maximal activity, pH 10.0: about 65% of maximal activity Arthrobacter sp. TYUT010-15

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 20°C, 24 h, more than 80% residual activity Arthrobacter sp. TYUT010-15

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
 (1S,2S)-2-aminocyclohexanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
1.7
-
 (1S,2R)-1-amino-2-indanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
1.8
-
 (1S,2S)-2-aminocyclopentanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
4.5
-
 (1R,2R)-2-aminocyclohexanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
5.1
-
 (1R,2R)-2-aminocyclopentanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15
8.2
-
 (1R,2S)-1-amino-2-indanol pH 7.0, 30°C Arthrobacter sp. TYUT010-15