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Literature summary for 1.4.3.10 extracted from
- Oxidation of cadaverine by putrescine oxidase from Rhodococcus erythropolis (2021), J. Chem. Technol. Biotechnol., 96, 2950-2955 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | potential of putrescine oxidase for the bioproduction of N-heterocycles from cadaverine. Complete biotransformation of cadaverine is observed in whole cells at physiological conditions | Rhodococcus erythropolis |
| synthesis | potential of putrescine oxidase for the bioproduction of N-heterocycles from cadaverine | Rhodococcus erythropolis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no substrate inhibition at concentrations as high as 100 mM/l cadaverine | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | B0F9F6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cadaverine + O2 + H2O | - |
Rhodococcus erythropolis | 5-aminopentanal + NH3 + H2O2 | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Re-PuO | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Rhodococcus erythropolis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 75 | 35°C: about 50% of maximal activity, 75°C: about 60% of maximal activity | Rhodococcus erythropolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
pH 7.5, the enzyme retains its activity after 65 h incubation | Rhodococcus erythropolis |
| 55 | - |
pH 7.5, the enzyme loses 75% of its activity after 1 h incubation at 55°C | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Rhodococcus erythropolis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | pH 7.5: about 60% of maximal activity, pH 9.5: about 80% of maximal activity | Rhodococcus erythropolis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
the enzyme retains its activity after 65 h incubation at 25°C, but lost 75% of its activity after 1 h incubation at 55°C | Rhodococcus erythropolis |
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Release 2023.1 (January 2023)
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