Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M sodium nitrate, 0.1 M Bis-Tris propane (pH 8.5) and 20% (w/v) PEG 3350 | Roseovarius nubinhibens |
Protein Variants | Comment | Organism |
---|---|---|
E435A | the mutation completely abolishes the activity of the enzyme | Roseovarius nubinhibens |
F195A | the mutation almost completely abolishes the activity of the enzyme to 0.4% of wild type activity | Roseovarius nubinhibens |
F287A | the mutant with 76.3% of wild type activity shows significantly increased Km to 3-(methylsulfanyl)propanoyl-CoA | Roseovarius nubinhibens |
H280A | the mutation severely reduces the activity of the enzyme to 18.2%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA | Roseovarius nubinhibens |
K223A | the mutation severely reduces the activity of the enzyme to 9.4%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA | Roseovarius nubinhibens |
K281A | the mutation severely reduces the activity of the enzyme to 54.5%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA | Roseovarius nubinhibens |
M161A | the mutation significantly decreases the activity of the enzyme to 37.5% and does not affect its Km value to FAD or 3-(methylsulfanyl)propanoyl-CoA | Roseovarius nubinhibens |
R284A | the mutant with 97.2% of wild type activity shows significantly increased Km to 3-(methylsulfanyl)propanoyl-CoA | Roseovarius nubinhibens |
R448A | the mutant with 44.9% of wild type activity shows significantly increased Km for 3-(methylsulfanyl)propanoyl-CoA | Roseovarius nubinhibens |
S197A | the mutation significantly decreases the activity of the enzyme to 3.6% and increases its Km value to FAD | Roseovarius nubinhibens |
T170A | the mutation significantly decreases the activity of the enzyme to 37.5% and increases its Km value to FAD | Roseovarius nubinhibens |
Y434A | the mutation significantly decreases the activity of the enzyme to 51.2% and increases its Km value to FAD but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA | Roseovarius nubinhibens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
FAD | wild type enzyme, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
0.0092 | - |
FAD | mutant enzyme M161A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
0.0299 | - |
FAD | mutant enzyme Y434A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
0.0648 | - |
FAD | mutant enzyme S197A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
0.1125 | - |
FAD | mutant enzyme T170A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
1.4 | - |
3-(methylsulfanyl)propanoyl-CoA | wild type enzyme, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
1.6 | - |
3-(methylsulfanyl)propanoyl-CoA | mutant enzyme Y434A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
2 | - |
3-(methylsulfanyl)propanoyl-CoA | mutant enzyme H280A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
2 | - |
3-(methylsulfanyl)propanoyl-CoA | mutant enzyme K223A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
6.3 | - |
3-(methylsulfanyl)propanoyl-CoA | mutant enzyme M161A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
8 | - |
3-(methylsulfanyl)propanoyl-CoA | mutant enzyme K281A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
12.4 | - |
3-(methylsulfanyl)propanoyl-CoA | mutant enzyme R284A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
14.3 | - |
3-(methylsulfanyl)propanoyl-CoA | mutant enzyme F287A, at pH 9.0 and 40°C | Roseovarius nubinhibens | |
100 | - |
3-(methylsulfanyl)propanoyl-CoA | Km above 100 mM, mutant enzyme R448A, at pH 9.0 and 40°C | Roseovarius nubinhibens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(methylsulfanyl)propanoyl-CoA + FAD | Roseovarius nubinhibens | - |
3-(methylsulfanyl)acryloyl-CoA + FADH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Roseovarius nubinhibens | A3SI50 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(methylsulfanyl)propanoyl-CoA + FAD | - |
Roseovarius nubinhibens | 3-(methylsulfanyl)acryloyl-CoA + FADH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 64210, calculated from amino acid sequence | Roseovarius nubinhibens |
Synonyms | Comment | Organism |
---|---|---|
dmdC | - |
Roseovarius nubinhibens |
MMPA-CoA dehydrogenase | - |
Roseovarius nubinhibens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Roseovarius nubinhibens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | more than 70% activity between 30 and 50°C | Roseovarius nubinhibens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Roseovarius nubinhibens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | more than 60% activity between pH 5.0 and 9.5 | Roseovarius nubinhibens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Roseovarius nubinhibens |
Organism | Comment | Expression |
---|---|---|
Roseovarius nubinhibens | the enzyme is up-regulated by dimethylsulfoniopropionate | up |