Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)Lys cells | Auxenochlorella protothecoides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Auxenochlorella protothecoides | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
49000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
58000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + reduced ferredoxin + ATP | Auxenochlorella protothecoides | - |
protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Auxenochlorella protothecoides | Q6VQA8 and Q6VQA9 and Q7YKW4 | subunit B, subunit L, and subunit N; strain CS-41, also named Auxenochlorella protothecoides strain CS-41 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + reduced ferredoxin + ATP | - |
Auxenochlorella protothecoides | protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
Synonyms | Comment | Organism |
---|---|---|
ChlB | subunit B | Auxenochlorella protothecoides |
ChlL | subunit L | Auxenochlorella protothecoides |
ChlN | subunit N | Auxenochlorella protothecoides |
light-independent protochlorophyllide oxidoreductase | - |
Auxenochlorella protothecoides |
light-independent protochlorophyllide reductase | - |
Auxenochlorella protothecoides |
LIPOR | - |
Auxenochlorella protothecoides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | the enzyme contains an Fe-S cluster as redox center in all subunits | Auxenochlorella protothecoides | |
ATP | subunit L contains the ATP-binding motif | Auxenochlorella protothecoides |
Organism | Comment | Expression |
---|---|---|
Auxenochlorella protothecoides | the level of theLIPOR subunit L mRNA of Chlorella protothecoides strain CS-41 is much higher in the light than in the dark | up |
General Information | Comment | Organism |
---|---|---|
physiological function | light-independent protochlorophyllide reductase is required for protochlorophyllide reduction in the dark | Auxenochlorella protothecoides |