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Literature summary for 1.3.5.1 extracted from

  • Singh, P.K.; Sarwar, M.; Maklashina, E.; Kotlyar, V.; Rajagukguk, S.; Tomasiak, T.M.; Cecchini, G.; Iverson, T.M.
    Plasticity of the quinone-binding site of the complex II homolog quinol fumarate reductase (2013), J. Biol. Chem., 288, 24293-24301 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.5.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
subunit FrdC mutant E29L, to 2.95 A resolution. The sequential removal of the two menaquinol protons may be accompanied by a rotation of the naphthoquinone ring to optimize the interaction with a second proton shuttling pathway Escherichia coli

Protein Variants

Protein Variants Comment Organism
E29L mutation in subunit FrdC, alters hydrogen bonding to menaquinone Escherichia coli
R28E/E29I mutation in subunit FrdC, retains normal level of activity Escherichia coli
R28L mutation in subunit FrdC, retains normal level of activity Escherichia coli
R28L/E9L mutation in subunit FrdC, retains normal level of activity Escherichia coli
R28L/R81A mutation in subunits FrdC/FrdD, loss of activtiy with ubiquinone and menaquinol Escherichia coli
R28N mutation in subunit FrdC, retains normal level of activity Escherichia coli
R81A mutation in subunit FrdD, retains normal level of activity Escherichia coli
R81E mutation in subunit FrdD, retains normal level of activity Escherichia coli
R81K mutation in subunit FrdD, retains normal level of activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 ubiquinone mutant R81E, pH 8.0, 30°C Escherichia coli
0.0006
-
 ubiquinone mutant R81A, pH 8.0, 30°C Escherichia coli
0.0009
-
 menaquinol mutant R81A, pH 7.0, 30°C Escherichia coli
0.0013
-
 menaquinol mutant R81E, pH 7.0, 30°C Escherichia coli
0.0013
-
 ubiquinone mutant R81K, pH 8.0, 30°C Escherichia coli
0.0015
-
 ubiquinone mutant R28L, pH 8.0, 30°C Escherichia coli
0.0015
-
 ubiquinone mutant R28N, pH 8.0, 30°C Escherichia coli
0.002
-
 ubiquinone mutant R28L/E29L, pH 8.0, 30°C Escherichia coli
0.0023
-
 ubiquinone wild-type, pH 8.0, 30°C Escherichia coli
0.0027
-
 menaquinol mutant R81K, pH 7.0, 30°C Escherichia coli
0.003
-
 menaquinol mutant R28L, pH 7.0, 30°C Escherichia coli
0.0031
-
 menaquinol mutant R28N, pH 7.0, 30°C Escherichia coli
0.0038
-
 ubiquinone mutant R28E/E29I, pH 8.0, 30°C Escherichia coli
0.0039
-
 menaquinol mutant R28L/E29L, pH 7.0, 30°C Escherichia coli
0.004
-
 menaquinol mutant R28E/E29I, pH 7.0, 30°C Escherichia coli
0.0054
-
 menaquinol wild-type, pH 7.0, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P00363 and P0A8Q0 and P0A8Q3 P00363 i.e. subunit FrdA, P0A8Q0 i.e. subunit FrdC, P0A8Q3 i.e. subunit FrdD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + menaquinol
-
 Escherichia coli succinate + menaquinone
-
 ?
succinate + ferricyanide
-
 Escherichia coli fumarate + ferrocyanide
-
 ?
succinate + ubiquinone
-
 Escherichia coli fumarate + ubiquinol
-
 ?

Synonyms

Synonyms Comment Organism
FrdA flavoprotein subunit Escherichia coli
FrdC
-
 Escherichia coli
FrdD
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 ubiquinone mutant R28E/E29I, pH 8.0, 30°C Escherichia coli
2.8
-
 menaquinol mutant R28E/E29I, pH 8.0, 30°C Escherichia coli
3
-
 menaquinol mutant R28E/E29I, pH 7.0, 30°C Escherichia coli
6.1
-
 menaquinol mutant R81A, pH 8.0, 30°C Escherichia coli
10.6
-
 menaquinol mutant R81A, pH 7.0, 30°C Escherichia coli
10.7
-
 ubiquinone mutant R81E, pH 8.0, 30°C Escherichia coli
11.1
-
 menaquinol mutant R28L/E29L, pH 8.0, 30°C Escherichia coli
12.2
-
 menaquinol mutant R28L/E29L, pH 7.0, 30°C Escherichia coli
12.8
-
 menaquinol mutant R81E, pH 8.0, 30°C Escherichia coli
13.9
-
 ubiquinone mutant R28L/E29L, pH 8.0, 30°C Escherichia coli
14
-
 menaquinol mutant R81E, pH 7.0, 30°C Escherichia coli
17.4
-
 ubiquinone mutant R81A, pH 8.0, 30°C Escherichia coli
19
-
 ubiquinone mutant R28N, pH 8.0, 30°C Escherichia coli
19.3
-
 ubiquinone mutant R28L, pH 8.0, 30°C Escherichia coli
20.1
-
 ferricyanide mutant R28L/R81A, pH 8.0, 30°C Escherichia coli
24.6
-
 ferricyanide mutant R28N, pH 8.0, 30°C Escherichia coli
24.9
-
 ubiquinone wild-type, pH 8.0, 30°C Escherichia coli
26.4
-
 ferricyanide mutant R28E/E29I, pH 8.0, 30°C Escherichia coli
27.1
-
 ferricyanide mutant R28L, pH 8.0, 30°C Escherichia coli
27.3
-
 ferricyanide mutant R81E, pH 8.0, 30°C Escherichia coli
28.1
-
 ferricyanide mutant R81A, pH 8.0, 30°C Escherichia coli
28.7
-
 ferricyanide wild-type, pH 8.0, 30°C Escherichia coli
28.9
-
 ferricyanide mutant R28L/E29L, pH 8.0, 30°C Escherichia coli
30.4
-
 ferricyanide mutant R81K, pH 8.0, 30°C Escherichia coli
32.2
-
 ubiquinone mutant R81K, pH 8.0, 30°C Escherichia coli
65.4
-
 menaquinol mutant R28N, pH 8.0, 30°C Escherichia coli
67.8
-
 menaquinol mutant R28L, pH 8.0, 30°C Escherichia coli
68.6
-
 menaquinol mutant R28N, pH 7.0, 30°C Escherichia coli
71.1
-
 menaquinol mutant R28L, pH 7.0, 30°C Escherichia coli
123.7
-
 menaquinol mutant R81K, pH 7.0, 30°C Escherichia coli
138.9
-
 menaquinol mutant R81K, pH 8.0, 30°C Escherichia coli
211
-
 menaquinol wild-type, pH 8.0, 30°C Escherichia coli
222.2
-
 menaquinol wild-type, pH 7.0, 30°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Escherichia coli
[3Fe-4S]-center located in subunit FrdC Escherichia coli