Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fumarate | - |
Wolinella succinogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2,3-dimethyl-1,4-naphthohydroquinone | - |
Wolinella succinogenes | |
0.35 | - |
fumarate | donor menadiol | Wolinella succinogenes | |
20 | - |
succinate | - |
Wolinella succinogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | - |
Wolinella succinogenes | - |
- |
cytoplasmic membrane | - |
Wolinella succinogenes | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Wolinella succinogenes | |
Iron | - |
Wolinella succinogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
25000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
31000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
31000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
31000 | - |
1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
79000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
79000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
79000 | - |
1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
110000 | 114000 | excluding cytochrome b, calculation from FAD-content and subunit composition | Wolinella succinogenes |
147000 | - |
calculation from Stokes radius and sedimentation coefficient | Wolinella succinogenes |
160000 | 167000 | including cytochrome b, calculation from FAD-content and subunit composition | Wolinella succinogenes |
200000 | - |
including cytochrome b, sedimentation equilibrium centrifugation | Wolinella succinogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wolinella succinogenes | - |
formerly Vibrio succinogenes, fumarate reductase | - |
Purification (Comment) | Organism |
---|---|
- |
Wolinella succinogenes |
using solubilization with Triton X-100, chromatography on hydroxyapatite column and DEAE-Sephadex column, the enzyme elutes from the ion-exchange column in two forms, one containing and the other lacking cytochrome b | Wolinella succinogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
- |
Wolinella succinogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + 2,3-dimethyl-1,4-naphthohydroquinone | cytochrome b-dependent in both directions | Wolinella succinogenes | succinate + 2,3-dimethyl-1,4-naphthoquinone | - |
r | |
fumarate + anthrahydroquinonesulfonate | reaction catalyzed in the presence or absence of cytochrome b | Wolinella succinogenes | succinate + anthraquinonesulfonate | - |
? | |
fumarate + electron donor | donor: anthrahydroquinonesulfonate | Wolinella succinogenes | succinate + oxidized donor | - |
r | |
succinate + electron acceptor | acceptor: methylene blue (oxidized) | Wolinella succinogenes | fumarate + reduced acceptor | - |
r | |
succinate + electron acceptor | acceptor: ferricyanide | Wolinella succinogenes | fumarate + reduced acceptor | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
tetramer | 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
trimer | enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b | 2 mol per mol FAD | Wolinella succinogenes | |
cytochrome b | 2 mol per mol of FAD | Wolinella succinogenes | |
FAD | - |
Wolinella succinogenes | |
FAD | one protein-bound FAD linked to the 79000 Da peptide | Wolinella succinogenes | |
additional information | the enzyme contains iron-sulfur centers | Wolinella succinogenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
fumarate | - |
Wolinella succinogenes |