Crystallization (Comment) | Organism |
---|---|
sitting drop method, structure of TyrAsy in complex with NADP+ is refined to 1.6 A. The asymmetric unit contains two TyrAsy homodimers, with each monomer consisting of a nucleotide binding N-terminal domain and a particularly unique alpha-helical C-terminal dimerization domain | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | almost completely insensitive to feedback inhibition by tyrosine. Phenylalanine and tryptophan show no effect at concentrations of up to 1 mM | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
NADP+ | - |
Synechocystis sp. | |
0.093 | - |
L-arogenate | - |
Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Synechocystis sp. |
70000 | - |
gel filtration | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
88.1 | - |
- |
Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arogenate + NADP+ | - |
Synechocystis sp. | L-tyrosine + NADPH + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
TyrAsy | - |
Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Synechocystis sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | enzyme activity dropps below pH 6.5 and above pH 8.5 | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | no activity with NAD+ | Synechocystis sp. |