KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
2,3-dihydro-2,3,-dihydroxybiphenyl | activity measured with 1 micromol NAD+ in the reaction mixture at pH 9.5 and 37°C | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
123000 | - |
native protein, gel filtration | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+ | Comamonas testosteroni | biphenyl degradation pathway | 2,3-dihydroxybiphenyl + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
gene bphB | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46 | - |
- |
Comamonas testosteroni |
Storage Stability | Organism |
---|---|
-70°C for months | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+ | - |
Comamonas testosteroni | 2,3-dihydroxybiphenyl + NADH | - |
? | |
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+ | biphenyl degradation pathway | Comamonas testosteroni | 2,3-dihydroxybiphenyl + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Comamonas testosteroni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
recombinant His-tagged enzyme | Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | essential cofactor for His-taggged enzyme | Comamonas testosteroni |