Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition at 2 mM | Cannabis sativa | |
additional information | EDTA has little influence on the enzyme activity at concentrations up to 5 mM | Cannabis sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.137 | - |
cannabigerolate | in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa | |
0.206 | - |
cannabinerolate | in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require metal ions | Cannabis sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
1 * 74000, SDS-PAGE | Cannabis sativa |
75000 | - |
gel filtration | Cannabis sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cannabis sativa | - |
CBDA and Mexican strains | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose CL-4B column chromatography, and hydroxylapatite column chromatography | Cannabis sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Cannabis sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.175 | - |
enzyme from supernatant, in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa |
9 | - |
after 519fold purification, in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa |
Storage Stability | Organism |
---|---|
-20°C, 40% (v/v) glycerol, ineffective storage conditions for enzyme stabilization | Cannabis sativa |
4°C, purified enzyme in 10 mM sodium phosphate buffer (pH 7.0), 3 mM beta-mercaptoethanol, 2 weeks, 10-20% loss of activity | Cannabis sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cannabigerolate + O2 | best substrate | Cannabis sativa | cannabidiolate + H2O2 | - |
? | |
cannabinerolate + O2 | - |
Cannabis sativa | cannabidiolate + H2O2 | - |
? | |
additional information | the enzyme does not require molecular oxygen for the production of cannabidiolate in the presence or absence of NADPH | Cannabis sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 74000, SDS-PAGE | Cannabis sativa |
Synonyms | Comment | Organism |
---|---|---|
CBDA synthase | - |
Cannabis sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
cannabinerolate | in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa | |
0.19 | - |
cannabigerolate | in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Cannabis sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | half-maximal activities at pH values around 4.0 and 6.0 | Cannabis sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require any coenzyme like NAD+, NADP+, FAD, and FMN | Cannabis sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cannabis sativa | isoelectric focusing | - |
6.1 |