Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermoplasma acidophilum |
expressed in Escherichia coli | Desulfitobacterium hafniense |
expressed in Escherichia coli | Syntrophus aciditrophicus |
Crystallization (Comment) | Organism |
---|---|
nanodroplet vapor diffusion method, using 0.01 M nickel chloride, 20% (w/v) PEG MME 2000 and 0.1 M Tris pH 8.5 | Syntrophus aciditrophicus |
nanodroplet vapor diffusion method, using 0.2 M magnesium nitrate and 20% (w/v) PEG 3350 or 10% (w/v) PEG 8000, 0.2 M zinc acetate and 0.1 M MES pH 6.0 | Thermoplasma acidophilum |
nanodroplet vapor diffusion method, using 0.2 M MgCl2 and 20% (w/v) PEG 3350 | Desulfitobacterium hafniense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | contains nickel | Desulfitobacterium hafniense | |
Zn2+ | contains zinc | Thermoplasma acidophilum | |
Zn2+ | contains zinc | Desulfitobacterium hafniense | |
Zn2+ | contains zinc | Syntrophus aciditrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17400 | - |
calculated from amino acid sequence | Desulfitobacterium hafniense |
21500 | - |
calculated from amino acid sequence | Syntrophus aciditrophicus |
23100 | - |
calculated from amino acid sequence | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfitobacterium hafniense | B8FYU2 | subunit E | - |
Desulfitobacterium hafniense DCB-2 | B8FYU2 | subunit E | - |
Syntrophus aciditrophicus | Q2LQ23 | subunit E | - |
Syntrophus aciditrophicus SB | Q2LQ23 | subunit E | - |
Thermoplasma acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelating resin column chromatography | Thermoplasma acidophilum |
nickel-chelating resin column chromatography | Desulfitobacterium hafniense |
nickel-chelating resin column chromatography | Syntrophus aciditrophicus |
Synonyms | Comment | Organism |
---|---|---|
FmdE | - |
Thermoplasma acidophilum |
FmdE | - |
Desulfitobacterium hafniense |
FmdE | - |
Syntrophus aciditrophicus |
PF02663 | - |
Thermoplasma acidophilum |
PF02663 | - |
Desulfitobacterium hafniense |
PF02663 | - |
Syntrophus aciditrophicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Desulfitobacterium hafniense | calculated from amino acid sequence | - |
5.6 |
Thermoplasma acidophilum | calculated from amino acid sequence | - |
6.1 |
Syntrophus aciditrophicus | calculated from amino acid sequence | - |
6.2 |