Cloned (Comment) | Organism |
---|---|
gene gapN, construction of the mutant Escherichia coli strain MG1655DELTAgapA::gapN using the fusion of the gapN gene with a chloramphenicol resistance cassette resulting in plasmid pTrcgapN | Streptococcus mutans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the gapA gene, UniProt ID P0A9B2, EC 1.2.1.12, from Escherichia coli strain MG1655 is replaced by the gene gapN from Streptococcus mutans. The specific NADP+-GAPDH activity of the strain MG1655DgapA::gapN is 4.6times lower relative to strain MG1655DELTAgapA::gapN/pTrcgapN and no NAD+-GAPDH activity is detected. The specific NADP+-GAPDH activity levels in the derivative strain reveal that growth rate and glucose uptake differences are attributable to gapN expression level. The NADH/NAD+ ratio in the strain MG1655DELTAgapA::gapN/pTrcgapN decreases by 25% as compared to wild-type strain. In contrast, the NADPH/NADP+ ratio increases 2times indicating that the alteration in the turnover of NAD(P)H via glyceraldehyde 3-phosphate oxidation affects the redox levels of the strain MG1655DELTAgapA::gapN/pTrcgapN, which increases 2.8times the NADPH/NADH ratio | Streptococcus mutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O | Streptococcus mutans | - |
3-phospho-D-glycerate + NADPH + 2 H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | Q59931 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Streptococcus mutans | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GAPN | - |
Streptococcus mutans |
NADP+-dependent GAPDH | - |
Streptococcus mutans |
NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase | - |
Streptococcus mutans |
NADP+-GAPDH | - |
Streptococcus mutans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Streptococcus mutans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Streptococcus mutans | |
NADPH | - |
Streptococcus mutans |
General Information | Comment | Organism |
---|---|---|
metabolism | the EMP pathway can be controlled through the glyceraldehyde 3-phosphate node by NAD+-GAPDH activity, recombinant NADP+-GAPDH heterologous activity can also exert a similar response, which modulates the glucose uptake and also the acetic acid production rate | Streptococcus mutans |