Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-naphthoaldehyde | 0.2 mM, 34.6% inhibition | Alcaligenes faecalis | |
4-carboxybenzaldehyde | 0.2 mM, 38.5% inhibition | Alcaligenes faecalis | |
acetaldehyde | 0.2 mM, 34.6% inhibition | Alcaligenes faecalis | |
Ag+ | 1 mM, complete inhibition | Alcaligenes faecalis | |
Co2+ | 1 mM, 40.2% inhibition | Alcaligenes faecalis | |
Cu2+ | 1 mM, complete inhibition | Alcaligenes faecalis | |
Hg2+ | 1 mM, 17.5% inhibition | Alcaligenes faecalis | |
iso-phthalaldehyde | 0.2 mM, 23.8% inhibition | Alcaligenes faecalis | |
o-nitrobenzaldehyde | 0.2 mM, 58.1% inhibition | Alcaligenes faecalis | |
o-phthalaldehyde | 0.2 mM, 91.4% inhibition | Alcaligenes faecalis | |
p-chloromercuribenzoate | 1 mM, complete inhibition | Alcaligenes faecalis | |
p-dimethylamino benzaldehyde | 0.2 mM, 32.7% inhibition | Alcaligenes faecalis | |
propionaldehyde | 0.2 mM, 31.7% inhibition | Alcaligenes faecalis | |
Protocatechualdehyde | 0.2 mM, 23.1% inhibition | Alcaligenes faecalis | |
Salicylaldehyde | 0.2 mM, 23.1% inhibition | Alcaligenes faecalis | |
SDS | 1 mM, 44.3% inhibition | Alcaligenes faecalis | |
vertraldehyde | 0.2 mM, 13.5% inhibition | Alcaligenes faecalis | |
Zn2+ | 1 mM, 71.7% inhibition | Alcaligenes faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
2-carboxybenzaldehyde | pH 8.0, 30°C | Alcaligenes faecalis | |
0.14 | - |
NAD+ | pH 8.0, 30°C | Alcaligenes faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
4 * 41000, SDS-PAGE | Alcaligenes faecalis |
158000 | - |
gel filtration | Alcaligenes faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-carboxybenzaldehyde + NAD+ + H2O | Alcaligenes faecalis | the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene | o-phthalic acid + NADH + H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | Alcaligenes faecalis AFK2 | the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene | o-phthalic acid + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Alcaligenes faecalis AFK2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:phenanthrene-grown cell | - |
Alcaligenes faecalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130.6 | - |
- |
Alcaligenes faecalis |
Storage Stability | Organism |
---|---|
4°C, activity in phosphate buffer is labile, 1 mM DTT stabilizes | Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-carboxybenzaldehyde + NAD+ + H2O | the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene | Alcaligenes faecalis | o-phthalic acid + NADH + H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor | Alcaligenes faecalis | o-phthalic acid + NADH + H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene | Alcaligenes faecalis AFK2 | o-phthalic acid + NADH + H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor | Alcaligenes faecalis AFK2 | o-phthalic acid + NADH + H+ | - |
? | |
additional information | no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde | Alcaligenes faecalis | ? | - |
? | |
additional information | no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde | Alcaligenes faecalis AFK2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, SDS-PAGE | Alcaligenes faecalis |
Synonyms | Comment | Organism |
---|---|---|
2CBAL dehydrogenase | - |
Alcaligenes faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Alcaligenes faecalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
10 min, no loss of activity below 30°C | Alcaligenes faecalis |
40 | - |
10 min, 25% loss of activity | Alcaligenes faecalis |
50 | - |
10 min, 94% loss of activity | Alcaligenes faecalis |
60 | - |
10 min, complete loss of activity | Alcaligenes faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Alcaligenes faecalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.6 | pH 7.0: about 60% of maximal activity, pH 8.6: about 70% of maximal activity | Alcaligenes faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | high specificity for NAD+ as electron acceptor | Alcaligenes faecalis |