General Stability | Organism |
---|---|
glycerol in chromatographic buffers stabilizes | Nocadia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
anthranilic acid | competitive inhibitor with benzoic acid as a substrate | Nocadia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0293 | - |
NADPH | - |
Nocadia sp. | |
0.0345 | - |
(R)-ibuprofen | - |
Nocadia sp. | |
0.0573 | - |
ATP | - |
Nocadia sp. | |
0.155 | - |
Ibuprofen | - |
Nocadia sp. | |
0.645 | - |
benzoate | - |
Nocadia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
1 * 140000, SDS-PAGE | Nocadia sp. |
163000 | - |
gel filtration | Nocadia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocadia sp. | - |
- |
- |
Nocadia sp. NRRL 5646 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by a combination of Mono-Q, Reactive Green 19 agarose affinity chromatography, and hydroxyapatite chromatography | Nocadia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.89 | - |
purified enzyme | Nocadia sp. |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl buffer, pH 7.5, 10% glycerol, 8 hours | Nocadia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-ibuprofen + NADPH + ATP | - |
Nocadia sp. | (2R)-2-(4-(2-methylpropyl)phenyl)propanal + NADP+ + AMP + H2O | - |
? | |
(R)-ibuprofen + NADPH + ATP | - |
Nocadia sp. NRRL 5646 | (2R)-2-(4-(2-methylpropyl)phenyl)propanal + NADP+ + AMP + H2O | - |
? | |
aromatic acids + NADPH + H+ + ATP | - |
Nocadia sp. | aromatic aldehydes + NADP+ + AMP + diphosphate + H2O | - |
ir | |
aromatic acids + NADPH + H+ + ATP | - |
Nocadia sp. NRRL 5646 | aromatic aldehydes + NADP+ + AMP + diphosphate + H2O | - |
ir | |
benzoic acid + NADPH + H+ + ATP | the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde | Nocadia sp. | benzaldehyde + NADP+ + AMP + diphosphate | - |
? | |
benzoic acid + NADPH + H+ + ATP | the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde | Nocadia sp. NRRL 5646 | benzaldehyde + NADP+ + AMP + diphosphate | - |
? | |
ibuprofen + NADPH + ATP | - |
Nocadia sp. | 2-(4-isobutylphenyl)propanal + NADP+ + AMP + H2O | - |
? | |
ibuprofen + NADPH + ATP | - |
Nocadia sp. NRRL 5646 | 2-(4-isobutylphenyl)propanal + NADP+ + AMP + H2O | - |
? | |
additional information | other substrates are phenyl-substituted aliphatic acids, heterocyclic carboxylic acids, polyaromatic ring carboxylic acids, ibuprofen and its (R)-(-) isomer | Nocadia sp. | ? | - |
? | |
additional information | other substrates are phenyl-substituted aliphatic acids, heterocyclic carboxylic acids, polyaromatic ring carboxylic acids, ibuprofen and its (R)-(-) isomer | Nocadia sp. NRRL 5646 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 140000, SDS-PAGE | Nocadia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nocadia sp. | |
NADPH | - |
Nocadia sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.261 | - |
anthranilic acid | - |
Nocadia sp. |