Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 1 mM, isozyme A, 26% inhibition | Escherichia coli | |
As3+ | 1 mM, isozyme A, 42% inhibition, isozyme B, 11% inhibition, isozyme C, 55% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3 | Escherichia coli | |
Ca2+ | 1 mM, isozyme A, 42% inhibition, isozyme B, 32% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3 | Escherichia coli | |
Co2+ | 1 mM, isozyme A, 71% inhibition, isozyme B, 51% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3 | Escherichia coli | |
Fe2+ | 1 mM, isozyme A, 18% inhibition | Escherichia coli | |
HgCl2 | 1 mM, complete inhibition of isozymes A, B, and C | Escherichia coli | |
KCN | isozymes A and C | Escherichia coli | |
Mg2+ | 1 mM, isozyme A, 33% inhibition, isozyme B, 54% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3 | Escherichia coli | |
Mn2+ | 1 mM, isozyme A, 20% inhibition, isozyme C, 44% inhibition | Escherichia coli | |
monoiodoacetate | 1 mM, isozymes A, 83% inhibition, isozyme C, 100% inhibition | Escherichia coli | |
MoO22- | 1 mM, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3 | Escherichia coli | |
Zn2+ | 1 mM, isozyme A, 30% inhibition | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
isozyme B, gel filtration | Escherichia coli |
225000 | - |
isozyme A, gel filtration | Escherichia coli |
345000 | - |
isozyme C, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B WG1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial: isozymes A, B and C | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.7 | - |
isozyme B | Escherichia coli |
2.75 | - |
isozyme C | Escherichia coli |
3.66 | - |
isozyme A | Escherichia coli |
6.38 | - |
isozyme A from pyridoxine auxotroph strain WG3 | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74% | Escherichia coli | acetate + NADH + H+ | - |
r | |
acetaldehyde + NAD+ + H2O | reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74% | Escherichia coli B WG1 | acetate + NADH + H+ | - |
r | |
benzaldehyde + NAD+ + H2O | isozyme A, 195% of activity with glycolate in reverse reaction | Escherichia coli | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | isozyme A, 195% of activity with glycolate in reverse reaction | Escherichia coli B WG1 | benzoate + NADH + H+ | - |
? | |
betaine + NADH | isozyme A 139% of activity with glycolate, isozyme B, 76% | Escherichia coli | betaine aldehyde + NAD+ + H2O | glucono-delta-lactone is not used as substrate | ? | |
formate + NADH | isozyme A, 163% of activity with glycolate | Escherichia coli | methanal + NAD+ | - |
? | |
glycolaldehyde + NAD+ + H2O | - |
Escherichia coli | glycolate + NADH | - |
r | |
glycolaldehyde + NAD+ + H2O | - |
Escherichia coli B WG1 | glycolate + NADH | - |
r | |
glyoxylate + NADH | isozyme A, 417% of activity with glycolate, isozyme B 714%, isozyme C 150% | Escherichia coli | glyoxal + NAD+ | - |
? | |
malonate + NADH | isozyme A, 83% of activity with glycolate | Escherichia coli | malonic semialdehyde + NAD+ + H2O | - |
? | |
oxalate + NADH | isozyme A, 167% of activity with glycolate, isozyme B, 71% | Escherichia coli | glyoxylate + NAD+ | - |
? | |
oxalate + NADH | isozyme A, 167% of activity with glycolate, isozyme B, 71% | Escherichia coli B WG1 | glyoxylate + NAD+ | - |
? | |
succinate + NADH | isozyme A, 146% of activity with glycolate, isozyme B 6% | Escherichia coli | succinic semialdehyde + NAD+ + H2O | - |
? | |
succinate + NADH | isozyme A, 146% of activity with glycolate, isozyme B 6% | Escherichia coli B WG1 | succinic semialdehyde + NAD+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
isozyme A | Escherichia coli |
30 | - |
isozyme A from strain WG3 | Escherichia coli |
45 | - |
isozymes B and C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
glycolate reduction, isozyme A, B | Escherichia coli |
5.4 | - |
isozyme A, glycolaldehyde oxidation | Escherichia coli |
6 | - |
glycolate reduction, isozyme C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli |