General Stability | Organism |
---|---|
2-mercaptoethanol and NAD+, stabilization | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
A.T.C.C. 13430 | - |
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using protamine, ammonium sulfate, acetone and column chromatography on DEAE-cellulose | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanal + NAD+ + H2O | - |
Pseudomonas fluorescens | 4-aminobutanoate + NADH + H+ | - |
? | |
4-aminobutanal + NAD+ + H2O | - |
Pseudomonas sp. | 4-aminobutanoate + NADH + H+ | - |
? | |
additional information | - |
Pseudomonas sp. | ? | - |
? | |
additional information | not: DELTA1-piperidine, glutamic semialdehyde, succinic semialdehyde, malonic semialdehyde | Pseudomonas fluorescens | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | - |
80% loss of activity after overnight storage | Pseudomonas fluorescens |
50 | - |
for 5 min, 63% activity recovered | Pseudomonas fluorescens |
50 | - |
for 5 min, NAD+ present during heating, 100% activity recovered | Pseudomonas fluorescens |
60 | - |
for 5 min, 33% activity recovered | Pseudomonas fluorescens |
60 | - |
for 5 min, NAD+ present during heating, 53% activity recovered | Pseudomonas fluorescens |
70 | - |
for 5 min, 3% activity recovered | Pseudomonas fluorescens |
70 | - |
for 5 min, NAD+ present during heating, 16% activity recovered | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas fluorescens | |
NAD+ | - |
Pseudomonas sp. |