Crystallization (Comment) | Organism |
---|---|
crystallization of the ternary complex between GAPDH, intrinsically disordered protein CP12 and NAD+ in both the absence and presence of copper. The oxidized CP12 proten becomes partially structured upon GAPDH binding. The C-terminus of CP12 is inserted into the active-site region of GAPDH, resulting in competitive inhibition of GAPDH | Synechococcus elongatus |
in complex with CP12 and NAD+, hanging drop vapor diffusion method, using 20% (w/v) PEG3350 and 0.2 M magnesium acetate | Synechococcus elongatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the C-terminus of CP12 is inserted into the active-site region of glyceraldehyde-3-phosphate dehydrogenase, resulting in competitive inhibition of the enzyme | Synechococcus elongatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36830 | - |
4 * 36830, MALDI-TOF mass spectrometry | Synechococcus elongatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Synechococcus elongatus | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Synechococcus elongatus PCC 7942 | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus elongatus | Q9R6W2 | - |
- |
Synechococcus elongatus PCC 7942 | Q9R6W2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Synechococcus elongatus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Synechococcus elongatus PCC 7942 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 36830, MALDI-TOF mass spectrometry | Synechococcus elongatus |
Synonyms | Comment | Organism |
---|---|---|
gap2 | - |
Synechococcus elongatus |
GAPDH | - |
Synechococcus elongatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Synechococcus elongatus |