Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cryptosporidium parvum |
Crystallization (Comment) | Organism |
---|---|
structure in absence and presence of NAD+ and structure of the ternary enzyme-cofoactor-substrate complex to 2.0 A resolution. The active site can accomodate the substrate in multiple conformations at multiple locations during the initial encounter. the C-3 phosphate group clearly prefers the so-called new phosphate site for initial binding | Cryptosporidium parvum |
Protein Variants | Comment | Organism |
---|---|---|
C153S | about 450fold decrease in activity | Cryptosporidium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000032 | - |
NAD+ | - |
Cryptosporidium parvum | |
0.762 | - |
D-glyceraldehyde 3-phosphate | - |
Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | Q7YYQ9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Cryptosporidium parvum | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |