Cloned (Comment) | Organism |
---|---|
gene lytB, expression as His-tagged enzyme in Escherichia coli strain XL1-Blue | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | recombinant enzyme, pH 7.5, 60°C | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Aquifex aeolicus | - |
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | Aquifex aeolicus | part of isoprenoid biosynthesis | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | Aquifex aeolicus | 2-C-methyl-D-erithritol-4-phosphate pathway | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67625 | gene lytB | - |
Oxidation Stability | Organism |
---|---|
highly sensitive to O2, 21% remaining activity after incubation in air for 10 min | Aquifex aeolicus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein from Escherichia coli strain Xl1-Blue, to 95% homogeneity | Aquifex aeolicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | reaction mechanism | Aquifex aeolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.6 | - |
recombinant enzyme | Aquifex aeolicus |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 95% remaining activity after 2 weeks | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Aquifex aeolicus | isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | producing a 5:1 mixture of isopentenyl diphosphate and dimethyallyl diphosphate | r | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Aquifex aeolicus | dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Aquifex aeolicus | dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | producing a 5:1 mixture of isopentenyl diphosphate and dimethyallyl diphosphate | r | |
isopentenyl diphosphate + NAD(P)+ + H2O | part of isoprenoid biosynthesis | Aquifex aeolicus | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | 2-C-methyl-D-erithritol-4-phosphate pathway | Aquifex aeolicus | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aquifex aeolicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | 72 | half-maximal activity at 48°C and 72°C | Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable | Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | recombinant enzyme, pH 7.5, 60°C | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Aquifex aeolicus |