Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) cells | Vitis vinifera |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour-diffusion method | Selaginella moellendorffii |
sitting-drop vapour-diffusion method | Neisseria meningitidis |
vapour-diffusion method | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | dihydrodipicolinate reductase enzymes from plants are much more prone to substrate inhibition than the bacterial enzymes, which appears to be a consequence of increased flexibility of the substrate-binding loop and higher affinity for the nucleotide substrate | Arabidopsis thaliana | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | dihydrodipicolinate reductase enzymes from plants are much more prone to substrate inhibition than the bacterial enzymes, which appears to be a consequence of increased flexibility of the substrate-binding loop and higher affinity for the nucleotide substrate | Neisseria meningitidis | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | dihydrodipicolinate reductase enzymes from plants are much more prone to substrate inhibition than the bacterial enzymes, which appears to be a consequence of increased flexibility of the substrate-binding loop and higher affinity for the nucleotide substrate | Selaginella moellendorffii | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | dihydrodipicolinate reductase enzymes from plants are much more prone to substrate inhibition than the bacterial enzymes, which appears to be a consequence of increased flexibility of the substrate-binding loop and higher affinity for the nucleotide substrate | Vitis vinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
NADH | pH 8.0, 25°C | Neisseria meningitidis | |
0.0014 | - |
NADH | pH 8.0, 25°C | Selaginella moellendorffii | |
0.0031 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Vitis vinifera | |
0.0047 | - |
NADH | pH 8.0, 25°C | Vitis vinifera | |
0.0068 | - |
NADPH | pH 8.0, 25°C | Selaginella moellendorffii | |
0.0092 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADPH | Vitis vinifera | |
0.012 | - |
NADPH | pH 8.0, 25°C | Neisseria meningitidis | |
0.012 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Selaginella moellendorffii | |
0.013 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Neisseria meningitidis | |
0.016 | - |
NADPH | pH 8.0, 25°C | Vitis vinifera | |
0.058 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADPH | Selaginella moellendorffii | |
0.085 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADPH | Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62900 | - |
calculated | Vitis vinifera |
64200 | - |
calculated | Selaginella moellendorffii |
113000 | - |
calculated | Neisseria meningitidis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | Neisseria meningitidis | - |
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | Selaginella moellendorffii | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | Arabidopsis thaliana | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | Vitis vinifera | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8LB01 | - |
- |
Neisseria meningitidis | Q9K1F1 | - |
- |
Selaginella moellendorffii | D8R6G2 | - |
- |
Vitis vinifera | F6HB41 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | - |
Arabidopsis thaliana | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | - |
Neisseria meningitidis | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | Selaginella moellendorffii | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | Arabidopsis thaliana | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | Vitis vinifera | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Selaginella moellendorffii | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Vitis vinifera | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Neisseria meningitidis | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Selaginella moellendorffii | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Vitis vinifera | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Neisseria meningitidis | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the dimer forms through the intra-strand interface. Unique secondary features in plant enzymes block tetramer assembly | Selaginella moellendorffii |
dimer | the dimer forms through the intra-strand interface. Unique secondary features in plant enzymes block tetramer assembly | Arabidopsis thaliana |
dimer | the dimer forms through the intra-strand interface. Unique secondary features in plant enzymes block tetramer assembly | Vitis vinifera |
tetramer | - |
Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
DapB | - |
Neisseria meningitidis |
DHDPR | - |
Selaginella moellendorffii |
DHDPR | - |
Arabidopsis thaliana |
DHDPR | - |
Vitis vinifera |
DHDPR | - |
Neisseria meningitidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Selaginella moellendorffii |
25 | - |
assay at | Arabidopsis thaliana |
25 | - |
assay at | Vitis vinifera |
25 | - |
assay at | Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Vitis vinifera | |
21 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADPH | Vitis vinifera | |
43 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADPH | Neisseria meningitidis | |
49 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Selaginella moellendorffii | |
63 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADPH | Selaginella moellendorffii | |
77 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Neisseria meningitidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Selaginella moellendorffii |
8 | - |
assay at | Arabidopsis thaliana |
8 | - |
assay at | Vitis vinifera |
8 | - |
assay at | Neisseria meningitidis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Selaginella moellendorffii | |
NADH | - |
Vitis vinifera | |
NADPH | - |
Selaginella moellendorffii | |
NADPH | - |
Vitis vinifera |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Selaginella moellendorffii | |
0.76 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Vitis vinifera | |
1.3 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADPH | Selaginella moellendorffii | |
1.8 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADPH | Vitis vinifera | |
5.5 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | pH 8.0, 25°C, cofactor: NADH | Neisseria meningitidis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | Selaginella moellendorffii |
metabolism | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | Arabidopsis thaliana |
metabolism | the enzyme catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants | Vitis vinifera |