Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) R3 Rosetta cells | Bartonella henselae |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor-diffusion, protein crystals are grown in conditions consisting of 5%(w/v) PEG 4000, 200 mM sodium acetate, 100 mM sodium citrate tribasic pH 5.5 and are shown to diffract to about 2.3 A resolution. They belong to space group P4(3)22, with unit cell parameters a = 109.38, b = 109.38, c = 176.95 A | Bartonella henselae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Bartonella henselae |
114000 | - |
size-exclusion chromatography | Bartonella henselae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | Bartonella henselae | the enzyme catalyzes the second committed step in the diaminopimelate/lysine anabolic pathways | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | Bartonella henselae Houston 1 | the enzyme catalyzes the second committed step in the diaminopimelate/lysine anabolic pathways | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bartonella henselae | Q6G2G3 | - |
- |
Bartonella henselae Houston 1 | Q6G2G3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bartonella henselae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | the enzyme catalyzes the second committed step in the diaminopimelate/lysine anabolic pathways | Bartonella henselae | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | the enzyme catalyzes the second committed step in the diaminopimelate/lysine anabolic pathways | Bartonella henselae Houston 1 | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 28000, SDS-PAGE | Bartonella henselae |
Synonyms | Comment | Organism |
---|---|---|
DapB | - |
Bartonella henselae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the second committed step in the diaminopimelate/lysine anabolic pathways | Bartonella henselae |