Crystallization (Comment) | Organism |
---|---|
mutant E803V, decrease in activity towards purine substrates is not due to large conformational change in the mutant protein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E803V | almost complete loss of activity with hypoxanthine, weak activity with xanthine, significant aldehyde oxidase activity | Homo sapiens |
R881M | almost complete loss of activity with xanthine, weak activity with hypoxanthine, significant aldehyde oxidase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0088 | - |
hypoxanthine | wild-type, pH 8.5, 25°C | Homo sapiens | |
0.0088 | - |
xanthine | wild-type, pH 8.5, 25°C | Homo sapiens | |
0.072 | - |
xanthine | mutant E803V, pH 8.5, 25°C | Homo sapiens | |
0.072 | - |
hypoxanthine | mutant R881M, pH 8.5, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
after expression in Escherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H+ + O2- + H2O | - |
Homo sapiens | xanthine + NADH + H2O2 | - |
? | |
xanthine + NAD+ + H2O | - |
Homo sapiens | urate + NADH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
hypoxanthine | mutant R881M, pH 8.5, 25°C | Homo sapiens | |
0.99 | - |
xanthine | wild-type, pH 8.5, 25°C | Homo sapiens | |
1.35 | - |
xanthine | mutant E803V, pH 8.5, 25°C | Homo sapiens | |
18.33 | - |
xanthine | wild-type, pH 8.5, 25°C | Homo sapiens | |
30 | - |
hypoxanthine | wild-type, pH 8.5, 25°C | Homo sapiens |