Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Amino-2,6-dihydroxypyrimidine | competitive inhibition of xanthine oxidation, Ki: 0.106 mM | Colias eurytheme | |
Leucopterin | competitive inhibition of xanthopterin oxidation, Ki: 0.0109 mM | Colias eurytheme | |
Urate | competitive inhibition of xanthine oxidation, Ki: 0.064 mM | Colias eurytheme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
Xanthopterin | cosubstrate: NAD+ | Colias eurytheme | |
0.0061 | - |
2-amino-4-hydroxypterin | body preparation, cosubstrate: NAD+ | Colias eurytheme | |
0.0071 | - |
adenine | cosubstrate: NAD+ | Colias eurytheme | |
0.0072 | - |
2-amino-4-hydroxypterin | wing preparation, co substrate: NAD+ | Colias eurytheme | |
0.0182 | - |
purine | cosubstrate: NAD+ | Colias eurytheme | |
0.0258 | - |
4-hydroxypyrazolo(3,4-d)pyrimidine | cosubstrate: NAD+ | Colias eurytheme | |
0.0451 | - |
hypoxanthine | co substrate: NAD+ | Colias eurytheme | |
0.061 | - |
xanthine | wing preparation, cosubstrate: NAD+ | Colias eurytheme | |
0.0647 | - |
xanthine | body preparation, cosubstrate: NAD+ | Colias eurytheme | |
0.1065 | - |
NAD+ | cosubstrate: hypoxanthine | Colias eurytheme | |
0.124 | - |
NAD+ | cosubstrate: xanthine | Colias eurytheme |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | 300000 | gel filtration | Colias eurytheme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | Colias eurytheme | i.e. pterin | isoxanthopterin + NADH | - |
? | |
hypoxanthine + NAD+ + H2O | Colias eurytheme | - |
xanthine + NADH + H+ | - |
? | |
purine + NAD+ + H2O | Colias eurytheme | - |
? + NADH | - |
? | |
xanthine + NAD+ + H2O | Colias eurytheme | - |
urate + NADH | - |
? | |
xanthopterin + NAD+ + H2O | Colias eurytheme | regulation of the pteridine pathway by competitive inhibition of reaction products and the precursor of xanthopterin, 7,8-dihydroxanthopterin | leucopterin + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colias eurytheme | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Colias eurytheme |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
wing | - |
Colias eurytheme | - |
Storage Stability | Organism |
---|---|
1°C, 1 mM DTT in conjunction with 0.1 M ammonium sulfate, 60-70% retaining activity after 8-12 days | Colias eurytheme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Colias eurytheme | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Colias eurytheme | isoxanthopterin + NADH | - |
? | |
4-hydroxypyrazolo(3,4-d)pyrimidine + NAD+ + H2O | best substrate tested | Colias eurytheme | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + NADH | - |
? | |
4-hydroxypyrazolo(3,4-d)pyrimidine + NAD+ + H2O | i.e. allopurinol | Colias eurytheme | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + NADH | - |
? | |
adenine + NAD+ + H2O | low activity | Colias eurytheme | ? + NADH | - |
? | |
hypoxanthine + NAD+ + H2O | - |
Colias eurytheme | xanthine + NADH + H+ | - |
? | |
purine + NAD+ + H2O | - |
Colias eurytheme | ? + NADH | - |
? | |
xanthine + NAD+ + H2O | - |
Colias eurytheme | urate + NADH | - |
? | |
xanthopterin + NAD+ + H2O | regulation of the pteridine pathway by competitive inhibition of reaction products and the precursor of xanthopterin, 7,8-dihydroxyxanthopterin | Colias eurytheme | leucopterin + NADH | - |
? | |
xanthopterin + NAD+ + H2O | regulation of the pteridine pathway by competitive inhibition of reaction products and the precursor of xanthopterin, 7,8-dihydroxanthopterin | Colias eurytheme | leucopterin + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
at 30°C | Colias eurytheme |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | stable within, loss of activity at more acidic pHs | Colias eurytheme |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Colias eurytheme |