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Literature summary for 1.14.19.79 extracted from

  • Ohnishi, T.; Godza, B.; Watanabe, B.; Fujioka, S.; Hategan, L.; Ide, K.; Shibata, K.; Yokota, T.; Szekeres, M.; Mizutani, M.
    CYP90A1/CPD, a brassinosteroid biosynthetic cytochrome P450 of Arabidopsis, catalyzes C-3 oxidation (2012), J. Biol. Chem., 287, 31551-31560 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.79

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00096
-
 (22S)-22-hydroxycampesterol at pH 7.3 and 30°C Arabidopsis thaliana
0.0019
-
 (22R,23R)-22,23-dihydroxycampesterol at pH 7.3 and 30°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q42569 ecotype Columbia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(22R,23R)-22,23-dihydroxycampesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (22R,23R)-22,23-dihydroxycampest-4-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(22S)-22-hydroxycampesterol + [reduced NADPH-hemoprotein reductase] + O2 preferred reaction Arabidopsis thaliana (22S)-22-hydroxycampest-4-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
6-deoxocathasterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (22S)-22-hydroxy-5alpha-campestan-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
6-deoxoteasterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana 3-dehydro-6-deoxoteasterone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
additional information no activity with cathasterone, campestanol, campesterol, (22S)-22-hydroxycampest-4-en-3-one, (22S)-22-hydroxy-5alpha-campestan-3-one, 3-epi-6-deoxocathasterone, 3-dehydro-6-deoxoteasterone, 6-deoxotyphasterol, 6-deoxocastasterone, castasterone, and brassinolide Arabidopsis thaliana ?
-
-

Synonyms

Synonyms Comment Organism
3beta,22-dihydroxysteroid 3-dehydrogenase
-
 Arabidopsis thaliana
CPD
-
 Arabidopsis thaliana
CYP90A1
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0018
-
 (22R,23R)-22,23-dihydroxycampesterol at pH 7.3 and 30°C Arabidopsis thaliana
0.012
-
 (22S)-22-hydroxycampesterol at pH 7.3 and 30°C Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.001
-
 (22R,23R)-22,23-dihydroxycampesterol at pH 7.3 and 30°C Arabidopsis thaliana
0.013
-
 (22S)-22-hydroxycampesterol at pH 7.3 and 30°C Arabidopsis thaliana