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Literature summary for 1.14.18.4 extracted from
- An oleate hydroxylase from the fungus Claviceps purpurea: cloning, functional analysis, and expression in Arabidopsis (2008), Plant Physiol., 147, 1325-1333.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | CpFAH, thet D12 oleate hydroxylase of nonplant origin, is a good candidate for the transgenic production of hydroxyl fatty acids in oilseed crops | Claviceps purpurea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| FAH gene, unrooted phylogenetic analysis, functional expression in Saccharomyces cerevisiae, expression in transgenic Arabidopsis thaliana plants under control of a seed-specific promoter | Claviceps purpurea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Claviceps purpurea | biosynthesis of ricinoleic acid in Claviceps purpurea is catalyzed by the fungal desaturase-like hydroxylase | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Claviceps purpurea | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sclerotium | - |
Claviceps purpurea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linoleic acid + ferrocytochrome P-450 + O2 | - |
Claviceps purpurea | linolenic acid + ferricytochrome P-450 + H2O | - |
? | |
| additional information | biosynthesis of ricinoleic acid in Claviceps purpurea is catalyzed by the fungal desaturase-like hydroxylase | Claviceps purpurea | ? | - |
? | |
| additional information | the enzyme acts predominantly as a hydroxylase, introducing hydroxyl groups at the 12-position of oleic acid and palmitoleic acid. It also shows DELTA12 desaturase activities on 16C and 18C monounsaturated fatty acids and, to a much lesser extent, omega3 desaturase activities on ricinoleic acid, overview | Claviceps purpurea | ? | - |
? | |
| oleate + ferrocytochrome P-450 + H+ + O2 | - |
Claviceps purpurea | 12-hydroxy-oleate + ferricytochrome P-450 + H2O | - |
? | |
| palmitoleate + ferrocytochrome P-450 + H+ + O2 | - |
Claviceps purpurea | 12-hydroxypalmitoleate + ferricytochrome P-450 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CpFAH | - |
Claviceps purpurea |
| DELTA12 oleate hydroxylase | - |
Claviceps purpurea |
| oleate hydroxylase | - |
Claviceps purpurea |
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