Cloned (Comment) | Organism |
---|---|
the wild-type Tyr and mutant constructs are transiently expressed in human embryonic kidney (HEK) 293T cells for functional analysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
M374G | potential to delete the enzymatic activity of Tyr. Major effect on the active site: the packing density of this normally rigid environment is significantly lowered when the original amino acid is mutated to the smaller glycine because the missing side chain of G374 can neither anchor the loop nor orient the side chain of H367 | Mus musculus |
M374G/S375G | Tyr-GG double mutant, potential to delete the enzymatic activity of Tyr. The M374G/S375G mutation, designated Tyr-GG, replaces two residues present in mTyr by the equivalent residues in mTyrp1 and could potentially modify the enzymatic properties of the protein, when compared with wild-type Tyr. Analysis of the behaviour of the individual mutants M374G and S374G indicates that loss of enzymatic activity in Tyr-GG is mostly because of the M374G mutation | Mus musculus |
S375G | potential to delete the enzymatic activity of Tyr | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | the active site of the tyrosinase model shows the same structural conformation as in sTyr and ibCO, where two copper ions are coordinated by three histidines each, forming a binuclear type 3 copper site similar to that of the template structure | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
corresponds to the fully deglycosylated protein backbone. Western blot analysis | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P11344 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + O2 + AH2 | radioactive substrate L-[3,5-3H]-tyrosine, specific activity 50 Ci/mmol | Mus musculus | L-dopa + H2O + A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mTyr | - |
Mus musculus |
phenoloxidase | - |
Mus musculus |
tyr | - |
Mus musculus |
tyrosinase | - |
Mus musculus |