Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | noncompetitive inhibition | Brassica oleracea | |
Citric acid | noncompetitive inhibition | Brassica oleracea | |
EDTA | sodium salt of ethylenediaminetetraacetic acid, uncompetitive inhibition | Brassica oleracea | |
sodium sulphate | most effective inhibitor, noncompetitive inhibition | Brassica oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.34 | - |
catechol | polyphenol oxidase shows the best activity toward catechol and 4-methyl catechol | Brassica oleracea | |
21 | - |
4-methyl catechol | polyphenol oxidase shows the best activity toward catechol and 4-methyl catechol | Brassica oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51300 | - |
SDS-PAGE | Brassica oleracea |
57000 | - |
gel filtration | Brassica oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
var. botrytis italica | - |
Purification (Comment) | Organism |
---|---|
extracted and purified through (NH4)2SO4 precipitation, ion-exchange and gel filtration chromatography | Brassica oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
floret | - |
Brassica oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purification of PPO from broccoli florets. Specific activity of crude 800, ammonium sulfate precipitation 3058, DEAE 20425, G-50 31430, G-100 37953 U/min/mg protein | Brassica oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + 1/2 O2 | - |
Brassica oleracea | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
caffeic acid + 1/2 O2 | - |
Brassica oleracea | caffeoyl quinone + H2O | - |
? | |
catechol + 1/2 O2 | - |
Brassica oleracea | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + 1/2 O2 | - |
Brassica oleracea | chlorogenoquinone + H2O | - |
? | |
ferulic acid + O2 + AH2 | - |
Brassica oleracea | (2E)-3-(3,4-dihydroxy-5-methoxyphenyl)prop-2-enoic acid + H2O + A | - |
? | |
additional information | enzyme catalyzes two distinct reactions: the o-hydroxylation of monophenols to o-diphenols (acts like cresolase (E.C. 1.14.18.1.)) and the oxidation of o-diphenols to o-quinones (acts like catecholase (E.C. 1.10.3.2.)). No activity towards monophenols (tyrosine) and low activity - towards trihydroxyphenol-phloroglucinol | Brassica oleracea | ? | - |
? | |
phloroglucinol + 1/2 O2 | - |
Brassica oleracea | ? | - |
? | |
vanillin + O2 + AH2 | - |
Brassica oleracea | 3,4-dihydroxy-5-methoxybenzaldehyde + H2O + A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | acts like cresolase and catecholase | Brassica oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
the optimum pH for broccoli PPO is 5.7 with catechol and 4-methylcatechol as substrates | Brassica oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0317 | - |
Sodium sulfate | 4-methyl catechol as a substrate | Brassica oleracea | |
0.056 | - |
ascorbic acid | catechol as a substrate | Brassica oleracea | |
0.068 | - |
EDTA | catechol as a substrate | Brassica oleracea | |
0.074 | - |
Citric acid | 4-methyl catechol as a substrate | Brassica oleracea | |
0.082 | - |
Citric acid | catechol as a substrate | Brassica oleracea | |
0.082 | - |
Sodium sulfate | catechol as a substrate | Brassica oleracea | |
0.088 | - |
ascorbic acid | 4-methyl catechol as a substrate | Brassica oleracea | |
0.188 | - |
EDTA | 4-methyl catechol as a substrate | Brassica oleracea |