KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters for free and immobilized enzymes, determined according to the Michaelis-Menten equation, show a lower Km value for PPO-MAC400 than for the free enzyme, indicating higher affinity towards substrate, while PPO-MAC200 exhibits a higher Km value | Solanum tuberosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Solanum tuberosum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
PPO is immobilized onto MAC400 and MAC200 at various enzyme activities 50000, 100000, 200000, 300000 U/l, at pH 5-8, and at temperature ranging from 10 to 40°C. The optimum conditions for immobilization of PPO onto MAC400 and MAC200 are temperature 40°C, pH 5 and at PPO activity equal to 300000 U/l | Solanum tuberosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dopa + 1/2 O2 | - |
Solanum tuberosum | L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 + AH2 | - |
Solanum tuberosum | L-dopa + H2O + A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Solanum tuberosum |
PPO | - |
Solanum tuberosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the optimum conditions for immobilization of PPO onto MAC400 and MAC200 are temperature 40°C, pH 5 and at PPO activity equal to 300000 U/l | Solanum tuberosum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | PPO is immobilized onto MAC400 and MAC200 at various enzyme activities 50000, 100000, 200000, 300000 U/l, at pH 5-8, and at temperature ranging from 10 to 40°C | Solanum tuberosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
the optimum conditions for immobilization of PPO onto MAC400 and MAC200 are temperature 40°C, pH 5 and at PPO activity equal to 300000 U/l | Solanum tuberosum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | PPO is immobilized onto MAC400 and MAC200 at various enzyme activities 50000, 100000, 200000, 300000 U/l, at pH 5-8, and at temperature ranging from 10 to 40°C | Solanum tuberosum |