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Literature summary for 1.14.14.83 extracted from
- Functional expression of geraniol 10-hydroxylase reveals its dual function in the biosynthesis of terpenoid and phenylpropanoid (2011), J. Agric. Food Chem., 59, 4637-4643.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| co-expression of G10H with the NADPH-cytochrome P450 reductase from Arabidopsis thaliana in Spodoptera frugiperda Sf9 cell microsomes using the baculovirus transfection method, quantitative expression analysis | Catharanthus roseus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0158 | - |
geraniol | pH 8.0, 30°C, recombinant enzyme | Catharanthus roseus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Catharanthus roseus | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | Q8VWZ7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Catharanthus roseus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | CrG10H also catalyzed 3'-hydroxylation of the flavanone naringenin to form eriodictyol, a reaction of the flavonoid 3'-hydroxylase. Analysis of the enzyme reactions of the recombinant enzyme with naringenin, apigenin, kaempferol, pcoumaric acid, and ferulic acid, mass spectrometric product analysis, overview | Catharanthus roseus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G10H | - |
Catharanthus roseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Catharanthus roseus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.131 | - |
geraniol | pH 8.0, 30°C, recombinant enzyme | Catharanthus roseus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Catharanthus roseus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | G10H catalyzes the first committed step of the biosynthetic pathway of strictosidine and other terpenoid indole alkaloids, overview | Catharanthus roseus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000008 | - |
geraniol | pH 8.0, 30°C, recombinant enzyme | Catharanthus roseus | |
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Release 2023.1 (January 2023)
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