Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | 90000 | sedimentation equilibrium, gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-imidazoleacetate + NADH + O2 | Pseudomonas sp. | the enzyme is part of the histidine catabolic pathway in which imidazaloneacetate is converted to aspartic acid by way of formiminoaspartic acid | 5-hydroxy-4-imidazoleacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using protamine sulfate treatment, ammonium sulfate fractionation, chromatography on TEAE-cellulose column, calcium phosphate gel treatment and chromatography on DEAE-Sephadex column | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-imidazoleacetate + NADH + O2 | the enzyme is part of the histidine catabolic pathway in which imidazaloneacetate is converted to aspartic acid by way of formiminoaspartic acid | Pseudomonas sp. | 5-hydroxy-4-imidazoleacetate + NAD+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol per mol enzyme | Pseudomonas sp. | |
FAD | cannot be replaced by FMN or riboflavin | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. |