Literature summary for 1.14.11.78 extracted from

Del Rio Flores, A.; Kastner, D.; Du, Y.; Narayanamoorthy, M.; Shen, Y.; Cai, W.; Vennelakanti, V.; Zill, N.; Dell, L.; Zhai, R.; Kulik, H.; Zhang, W.
Probing the mechanism of isonitrile formation by a non-heme iron(II)-dependent oxidase/decarboxylase (2022), J. Am. Chem. Soc., 144, 5893-5901 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.369	-	(3R)-3-[(E)-(carboxymethylidene)amino]butanoate	pH not specified in the publication, temperature not specified in the publication	Streptomyces coeruleorubidus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coeruleorubidus	A0A3B6UEU3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-3-[(E)-(carboxymethylidene)amino]butanoate + 2-oxoglutarate + O2	-	Streptomyces coeruleorubidus	(3R)-3-(methylidyneazaniumyl)butanoate + succinate + 2 CO2 + 2 H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.365	-	(3R)-3-[(E)-(carboxymethylidene)amino]butanoate	pH not specified in the publication, temperature not specified in the publication	Streptomyces coeruleorubidus

General Information

General Information	Comment	Organism
metabolism	the ScoE reaction initiates with C5 hydroxylation of (R)-3-((carboxymethyl)amino)butanoic acid. The hydroxylated product undergoes dehydration presumably mediated by Tyr96 to synthesize the C5 imine in a trans configuration. (R)-3-isocyanobutanoic acid is finally generated through radical-based decarboxylation of the imine	Streptomyces coeruleorubidus

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Release 2023.1 (January 2023)