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Literature summary for 1.14.11.66 extracted from

  • Loh, Y.; Zhang, W.; Chen, X.; George, J.; Ng, H.
    Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells (2007), Genes Dev., 21, 2545-2557 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.66

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM4C, cloning of the Jmjd2c intronic DNA containing these Oct4-binding sites upstream of or downstream from a luciferase reporter to test for enhancer activity, robust enhancer activity is observed when the constructs is transfected into embryonic stem cells, real-time PCR expression analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of Jmjd2c knockout embryonic stem cells by RNAi assay, Jmjd2c depletion leads to embryonic stem cell differentiation, which is accompanied by a reduction in the expression of embryonic stem cell-specific genes and an induction of lineage marker genes. The same mutations that disrupt the in vitro Oct4/DNA interactions also abolished the enhancer activities. Knockdown of Jmjd1a does not appreciably affect Jmjd2c and vice versa Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatin
-
 Mus musculus 785
-
nucleus
-
 Mus musculus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 Mus musculus
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 [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 Mus musculus
-
 [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8VCD7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryonic stem cell feeder-free E14 mouse ES cells Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme is active on H3K9me3/me2 and H3K36me3/me2 (EC 1.14.11.69) substrates Mus musculus ?
-
 ?
[histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Mus musculus [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Mus musculus [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
H3K9Me3 demethylase
-
 Mus musculus
histone H3 Lys 9 demethylase
-
 Mus musculus
JMJD2C
-
 Mus musculus
Kdm4c
-
 Mus musculus

Expression

Organism Comment Expression
Mus musculus gene Jmjd2c is positively regulated by the ES cell transcription factor Oct4 up

General Information

General Information Comment Organism
malfunction Jmjd2c depletion leads to embryonic stem cell differentiation, which is accompanied by a reduction in the expression of embryonic stem cell-specific genes and an induction of lineage marker genes. The level of H3K36Me3 is not significantly affected by Jmjd2c depletion. Knockdown of Jmjd1a does not appreciably affect Jmjd2c and vice versa Mus musculus
metabolism expression of histone H3 Lys 9 demethylases Jmjd1a (EC 1.14.11.65) and Jmjd2c is positively correlated with the pluripotent state of ES and iPS cells. Jmjd1a and Jmjd2c regulate the global levels of H3K9Me2 and H3K9Me3, respectively Mus musculus
physiological function Jmjd2c acts as a positive regulator for Nanog, which encodes for a key transcription factor for self-renewal in ES cells. Jmjd2c is required to reverse the H3K9Me3 marks at the Nanog promoter region and consequently prevents transcriptional repressors HP1 and KAP1 from binding. Jmjd2c regulates expression of Nanog through demethylation of H3K9Me3, overview. The ES cell transcription circuitry is connected to chromatin modulation through H3K9 demethylation in pluripotent cells Mus musculus