Literature summary for 1.14.11.45 extracted from

Shi, F.; Fan, Z.; Zhang, S.; Wang, Y.; Tan, S.; Li, Y.
Optimization of ribosomal binding site sequences for gene expression and 4-hydroxyisoleucine biosynthesis in recombinant corynebacterium glutamicum (2020), Enzyme Microb. Technol., 140, 109622 .

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Application

Application	Comment	Organism
synthesis	improved synthesis of 4-HIL by ribosomal binding site engineering for gene expression in Corynebacterium glutamicum. To supply the cosubstrate 2-oxoglutarate at different levels, the OdhI gene is expressed using the ribosomal binding site sequences. The O2 supply is further enhanced in by overexpressing the Vgb gene. 4-HIL (up to 119.27 mM) is produced in the best strain. The synchronic supply of cosubstrates 2-oxoglutarate and O2 is critical for the high-yield production of 4-HIL	Bacillus thuringiensis

Organism

Organism	UniProt	Comment	Textmining
Bacillus thuringiensis	E2GIN1	-	-

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Release 2023.1 (January 2023)