Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D133C | the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme | Escherichia coli |
H131C | the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme | Escherichia coli |
H187C | the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains iron(II) | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-base-CH3 + 2-oxoglutarate + O2 | Escherichia coli | - |
DNA-base + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, SP Sepharose cation exchange column chromatography, and Mono-S cation exchange column chromatography | Escherichia coli |
Storage Stability | Organism |
---|---|
the iron(II)-bound AlkB is reasonably stable at 4°C in the presence of 10 mM 2-mercaptoethanol at concentrations up to 2 mM | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-base-CH3 + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-base + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli |